汉赛巴尔通体中组成型和诱导型绿色荧光蛋白的表达
Constitutive and inducible green fluorescent protein expression in Bartonella henselae.
作者信息
Lee A K, Falkow S
机构信息
Department of Microbiology and Immunology, Stanford University, Stanford, California 94305, USA.
出版信息
Infect Immun. 1998 Aug;66(8):3964-7. doi: 10.1128/IAI.66.8.3964-3967.1998.
Abstract
The green fluorescent protein (GFP) gene was expressed on a plasmid in B. henselae, and GFP-expressing bacteria were visualized by fluorescence microscopy. HEp-2 cells infected with GFP-expressing bacteria were separated from uninfected cells with a fluorescence activated cell sorter. Promoter fusions of B. henselae chromosomal DNA to gfp were examined by flow cytometry, and a B. henselae groEL promoter fusion which induced expression at 37 degreesC was isolated.
摘要
绿色荧光蛋白（GFP）基因在汉赛巴尔通体的质粒上表达，通过荧光显微镜观察到表达GFP的细菌。用荧光激活细胞分选仪将感染了表达GFP细菌的HEp-2细胞与未感染的细胞分离。通过流式细胞术检测汉赛巴尔通体染色体DNA与gfp的启动子融合情况，并分离出在37℃诱导表达的汉赛巴尔通体groEL启动子融合体。
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