Chang J H, Roh J Y, Je Y H, Park H W, Jin B R, Woo S D, Kang S K
Division of Applied Biology and Chemistry, College of Agriculture and Life Sciences, Seoul National University, Suwon, Korea.
Lett Appl Microbiol. 1998 May;26(5):387-90. doi: 10.1046/j.1472-765x.1998.00355.x.
A strain of Bacillus thuringiensis, STB-1, toxic against Spodoptera exigua, was isolated. Bacillus thuringiensis STB-1 produced bipyramidal inclusions and reacted with the H antiserum of B. thuringiensis ssp. kurstaki. The plasmid and protein profiles of B. thuringiensis STB-1 were compared with those of its reference strains, ssp. kurstaki and ssp. kenyae. To verify the gene type of B. thuringiensis STB-1, PCR analysis was performed with Spodoptera-specific cry gene primers. The result showed that B. thuringiensis STB-1, unlike its reference strains, had crylAa, crylAb, crylAc and crylE, suggesting that B. thuringiensis STB-1 was a unique strain with respect to gene type. In addition, B. thuringiensis STB-1 showed a high level of toxicity against both S. exigua and Bombyx mori, whereas B. thuringiensis ssp. kurstaki HD-1 or ssp. kenyae showed a high level of toxicity against only Bombyx mori or S. exigua, respectively.
分离出了对甜菜夜蛾有毒性的苏云金芽孢杆菌菌株STB - 1。苏云金芽孢杆菌STB - 1产生双锥形包涵体，并与苏云金芽孢杆菌库尔斯塔克亚种的H抗血清发生反应。将苏云金芽孢杆菌STB - 1的质粒和蛋白质图谱与其参考菌株库尔斯塔克亚种和肯尼亚亚种的图谱进行了比较。为了验证苏云金芽孢杆菌STB - 1的基因类型，使用针对夜蛾科昆虫的cry基因引物进行了PCR分析。结果表明，与参考菌株不同，苏云金芽孢杆菌STB - 1含有cry1Aa、cry1Ab、cry1Ac和cry1E基因，这表明苏云金芽孢杆菌STB - 1在基因类型方面是一个独特的菌株。此外，苏云金芽孢杆菌STB - 1对甜菜夜蛾和家蚕均表现出高毒性，而苏云金芽孢杆菌库尔斯塔克亚种HD - 1或肯尼亚亚种分别仅对家蚕或甜菜夜蛾表现出高毒性。
