苏云金芽孢杆菌kurstaki INA - 02的cryV基因编码蛋白的杀虫活性。

Insecticidal activity of the protein encoded by the cryV gene of Bacillus thuringiensis kurstaki INA-02.

作者信息

Sasaki J, Asano S, Iizuka T, Bando H, Lay B W, Hastowo S, Powell G K, Yamamoto T

机构信息

Faculty of Agriculture, Hokkaido University, Sapporo, Japan.

出版信息

Curr Microbiol. 1996 Apr;32(4):195-200. doi: 10.1007/s002849900035.

DOI:10.1007/s002849900035

PMID:8867460

Abstract

A new host specificity was discovered with the insecticidal protein encoded by the cryV gene. The cryV gene was cloned from the Bacillus thuringiensis kurstaki INA-02 strain, which was selected among a number of B. thuringiensis isolates because of its high activity against Spodoptera litura. Analyses by polymerase chain reaction (PCR) revealed that INA-02 contained the cryIA(a) and cryV genes. Since no Spodoptera activity was observed with B. thuringiensis sotto, which contained only cryIA(a), insecticidal activity of the protein encoded by the cryV gene was investigated with several insect species including S. litura. For bioassay, the cryV gene was highly expressed in an acrystalliferous B. thuringiensis strain, BT51. The CryV protein from BT51 was assayed against larvae of three lepidopteran species, Bombyx mori, S. litura, and Plutella xylostella. The protein was highly active against S. litura and P. xylostella, suggestive that the protein contributes to the unique activity of INA-02.

摘要

cryV基因编码的杀虫蛋白发现了一种新的宿主特异性。cryV基因是从苏云金芽孢杆菌库斯塔克亚种INA-02菌株中克隆出来的，该菌株是从众多苏云金芽孢杆菌分离株中挑选出来的，因为它对斜纹夜蛾具有高活性。聚合酶链反应（PCR）分析表明，INA-02含有cryIA(a)和cryV基因。由于仅含有cryIA(a)的苏云金芽孢杆菌 sotto未观察到对斜纹夜蛾的活性，因此用包括斜纹夜蛾在内的几种昆虫物种研究了cryV基因编码蛋白的杀虫活性。为了进行生物测定，cryV基因在无晶体的苏云金芽孢杆菌菌株BT51中高表达。用来自BT51的CryV蛋白对三种鳞翅目昆虫家蚕、斜纹夜蛾和小菜蛾的幼虫进行了测定。该蛋白对斜纹夜蛾和小菜蛾具有高活性，表明该蛋白促成了INA-02的独特活性。