Katagiri T, Shinozaki K
Laboratory of Plant Molecular Biology, Institute of Physical and Chemical Research (RIKEN), Tsukuba Life Science Center, Ibaraki, Japan.
Biochem Biophys Res Commun. 1998 Jul 9;248(1):87-92. doi: 10.1006/bbrc.1998.8914.
Phosphatidic acid phosphatase (PAP) is an enzyme involved in lipid metabolism. Diacylglycerol (DG) and phosphatidic acid (PA) are a substrate and a product of PAP, respectively, and function as second messengers in several signal transduction pathways in animals. To investigate the function of PAP in Saccharomyces cerevisiae, we analyzed changes in cellular phenotypes of a mutant that has a disrupted PAP gene. Two putative genes for PAP (ScPAP1 and ScPAP2) are in the S. cerevisiae genome. We generated a ScPAP1-null mutant and observed its cellular phenotypic changes. The ScPAP1-null mutant cells aggregated in liquid culture, and microscopical analyses showed that these mutant cells have an abnormal cell shape and abnormal cytokinesis during cell division. The ScPAP1 is possibly involved in cell growth and cytokinesis in S. cerevisiae. Yeast phosphatidic acid phosphatase is possibly involved in cell growth and cytokinesis.
磷脂酸磷酸酶（PAP）是一种参与脂质代谢的酶。二酰基甘油（DG）和磷脂酸（PA）分别是PAP的底物和产物，并且在动物的几种信号转导途径中作为第二信使发挥作用。为了研究PAP在酿酒酵母中的功能，我们分析了一个PAP基因被破坏的突变体的细胞表型变化。酿酒酵母基因组中有两个假定的PAP基因（ScPAP1和ScPAP2）。我们构建了一个ScPAP1缺失突变体并观察其细胞表型变化。ScPAP1缺失突变体细胞在液体培养中聚集，显微镜分析表明这些突变体细胞具有异常的细胞形状以及细胞分裂过程中的异常胞质分裂。ScPAP1可能参与酿酒酵母的细胞生长和胞质分裂。酵母磷脂酸磷酸酶可能参与细胞生长和胞质分裂。
