从亲脂性酵母糠秕马拉色菌中鉴定和克隆两种新型过敏原。
Identification and cloning of two novel allergens from the lipophilic yeast, Malassezia furfur.
作者信息
Yasueda H, Hashida-Okado T, Saito A, Uchida K, Kuroda M, Onishi Y, Takahashi K, Yamaguchi H, Takesako K, Akiyama K
机构信息
Clinical Research Center for Allergy and Rheumatology, National Sagamihara Hospital, 18-1 Sakuradai, Kanagawa, Sagamihara, 228-8522, Japan.
出版信息
Biochem Biophys Res Commun. 1998 Jul 20;248(2):240-4. doi: 10.1006/bbrc.1998.8944.
Two novel allergens, designated Mal f 2 and Mal f 3 according to the WHO/IUIS Allergen Nomenclature Subcommittee recommendation, were isolated from the lipophilic yeast Malassezia furfur cell extracts and the genes coding for those were cloned. Mal f 2 and Mal f 3 had apparent molecular weights of 21 kDa and 20 kDa, respectively, on SDS-PAGE under reducing conditions. The identified cDNA clone of Mal f 2 encoded an open reading frame of 177 amino acid residues. Fifty-one percent identity was found between the Mal f 2 and Mal f 3 sequences. Comparison of the Mal f 2 and Mal f 3 sequences with known protein sequences revealed that they had sequence homology with two peroxisomal membrane proteins of Candida boidinii and an Aspergillus fumigatus allergen, Asp f 3. In RAST, both Mal f 2- and Mal f 3-specific IgE antibodies could be detected in approximately 70 % of sera from M. furfur sensitized patients with atopic dermatitis.
根据世界卫生组织/国际免疫学会过敏原命名小组委员会的建议，从亲脂性酵母糠秕马拉色菌细胞提取物中分离出两种新型过敏原，分别命名为Mal f 2和Mal f 3，并克隆了编码它们的基因。在还原条件下进行的SDS-PAGE分析中，Mal f 2和Mal f 3的表观分子量分别为21 kDa和20 kDa。鉴定出的Mal f 2 cDNA克隆编码一个由177个氨基酸残基组成的开放阅读框。Mal f 2和Mal f 3序列之间有51%的同一性。将Mal f 2和Mal f 3序列与已知蛋白质序列进行比较后发现，它们与博伊丁假丝酵母的两种过氧化物酶体膜蛋白以及烟曲霉过敏原Asp f 3具有序列同源性。在放射变应原吸附试验（RAST）中，约70%的患有特应性皮炎的糠秕马拉色菌致敏患者血清中可检测到Mal f 2和Mal f 3特异性IgE抗体。
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