Sohn S J, Huh I H, Au W W, Heo M Y
National Institute of Toxicological Research, Korea Food and Drug Administration, 5 Nokbun-dong, Eunpyung-Ku, Seoul 122-020, South Korea.
Mutat Res. 1998 Jun 18;402(1-2):231-6. doi: 10.1016/s0027-5107(97)00302-3.
Using the Ames bacterial mutagenicity test and an in vivo micronucleus test, we investigated the antigenotoxic effect of galangin against the genotoxicity of N-methyl-N-nitrosourea (MNU). In the Ames assay, galangin showed an antimutagenic effect towards MNU-induced mutagenicity of Salmonella typhimurium TA 100. In mice, galangin showed an anticlastogenic effect against MNU-induced micronuclei in polychromatic erythrocytes in the MNPCE in mouse bone marrow cells. On the other hand, galangin is neither mutagenic nor clastogenic in both assays. Results from our in vitro and in vivo studies indicate that galangin is capable of suppressing the mutagenicity and clastogenicity of MNU. Therefore, galangin may be a useful chemopreventive agent against potential long-term health effects from genotoxic environmental agents.
我们使用艾姆斯细菌诱变性试验和体内微核试验，研究了高良姜素对N-甲基-N-亚硝基脲（MNU）遗传毒性的抗诱变作用。在艾姆斯试验中，高良姜素对MNU诱导的鼠伤寒沙门氏菌TA 100的诱变性表现出抗诱变作用。在小鼠中，高良姜素对MNU诱导的小鼠骨髓细胞多染红细胞中的微核表现出抗断裂作用。另一方面，在这两种试验中，高良姜素既不具有诱变性也不具有断裂性。我们的体外和体内研究结果表明，高良姜素能够抑制MNU的诱变性和断裂性。因此，高良姜素可能是一种有用的化学预防剂，可预防遗传毒性环境因子对健康的潜在长期影响。
