Heo M Y, Lee S J, Kwon C H, Kim S W, Sohn D H, Au W W
College of Pharmacy, Kangweon National University, Chuncheon, South Korea.
Mutat Res. 1994 Dec 1;311(2):225-9. doi: 10.1016/0027-5107(94)90180-5.
Galangin, a flavonoid derivative, was tested for its anticlastogenic effect against the induction of chromosome aberrations by bleomycin. For an in vitro assay, galangin (0, 2 x 10(-8), 2 x 10(-7), and 2 x 10(-6) M) was added to mouse spleen lymphocyte cultures together with bleomycin (3 microgram/ml) at 24 h after Con A initiation of cultures. In an in vivo/in vitro experiment, galangin (0, 0.1, 1, 10, and 100 mg/kg) was administered to mice orally twice with a 24-h interval. Mice were killed 8 h later. Spleen lymphocytes were isolated and cultures were made. Bleomycin (3 microgram/ml) was added to the mouse spleen lymphocyte cultures at 24 h after Con A initiation. Both in vitro and in vivo/in vitro cultures were harvested at 42 h after initiation. The harvested cells were used for cytogenetic analyses. The results showed that in vitro or in vivo treatment of lymphocytes with galangin suppressed the induction of chromosome aberrations by bleomycin in a galangin dose-dependent manner. The galangin doses used were non-clastogenic to cells. The data from our in vitro and in vivo/in vitro studies confirmed each other and indicate that galangin is an anticlastogenic agent. The in vivo/in vitro protocol may be a useful means to assay the chemoprotective effects of chemicals in humans.
高良姜素是一种黄酮类衍生物，针对其对博来霉素诱导染色体畸变的抗断裂效应进行了测试。对于体外试验，在刀豆蛋白A启动培养24小时后，将高良姜素（0、2×10⁻⁸、2×10⁻⁷和2×10⁻⁶ M）与博来霉素（3微克/毫升）一起添加到小鼠脾淋巴细胞培养物中。在体内/体外实验中，以24小时间隔给小鼠口服高良姜素（0、0.1、1、10和100毫克/千克）两次。8小时后处死小鼠。分离脾淋巴细胞并进行培养。在刀豆蛋白A启动培养24小时后，将博来霉素（3微克/毫升）添加到小鼠脾淋巴细胞培养物中。体外培养和体内/体外培养均在启动后42小时收获。收获的细胞用于细胞遗传学分析。结果表明，体外或体内用高良姜素处理淋巴细胞可抑制博来霉素诱导的染色体畸变，且呈高良姜素剂量依赖性。所用的高良姜素剂量对细胞无断裂作用。我们体外和体内/体外研究的数据相互印证，表明高良姜素是一种抗断裂剂。体内/体外实验方案可能是一种评估化学物质对人类化学保护作用的有用方法。
