Orpin C G, Huskisson N S, Ward P F
Parasitology. 1976 Aug;73(1):83-95. doi: 10.1017/s0031182000051350.
A particulate polysaccharide was isolated by differential centrifugation and alkali extraction from homogenates of the cestode Moniezia expansa. The polysaccharide had the structure of a glycogen. Its chemical properties, infra-red spectrum and optical rotation showed that it consisted of alpha-1,4- and alpha-1,6-linked glucopyranose units. Examination of the complex with iodine and the precipitate with concanavalin-A showed that the structure was highly branched. Oxidation with periodate and hydrolysis with alpha- and beta-amylase were used to measure mean chain lengths. For the particulate preparation the average chain length was 12-9 glucose units and the exterior and interior chain lengths were 9-0 and 2-9 units respectively. The particulate preparation had a very high sedimentation constant (s20, w = 910) with a smaller component at about s20, w = 600, but the alkali extracted material had an s20, w = 61 similar to that shown by alkali degradation of the particulate preparation. The morphology of the particulate material was similar to that of rat liver glycogen, alpha, beta- and possible gamma-particles being identified by electron microscopy. The alpha-particles were relatively stable under acidic conditions remaining intact down to pH 2-5. At pH 1-7 the alpha-particles dissociated into their constituent beta-particles with a consequent decrease in the opalescence of the solution. The nitrogen content of 0-9% was high for a glycogen.
通过差速离心和碱提取法，从扩展莫尼茨绦虫的匀浆中分离出一种颗粒状多糖。该多糖具有糖原的结构。其化学性质、红外光谱和旋光性表明它由α-1,4-和α-1,6-连接的吡喃葡萄糖单元组成。用碘络合物和伴刀豆球蛋白A沉淀进行检测，结果表明该结构高度分支。用过碘酸盐氧化以及用α-淀粉酶和β-淀粉酶水解来测定平均链长。对于颗粒制剂，平均链长为12 - 9个葡萄糖单元，外链长和内链长分别为9 - 0和2 - 9个单元。颗粒制剂具有非常高的沉降常数（s20,w = 910），在约s20,w = 600处有一个较小的组分，但碱提取物的s20,w = 61，与颗粒制剂碱降解后的情况相似。颗粒物质的形态与大鼠肝脏糖原相似，通过电子显微镜鉴定出α、β以及可能的γ颗粒。α颗粒在酸性条件下相对稳定，在pH值低至2.5时仍保持完整。在pH 1.7时，α颗粒解离成其组成的β颗粒，溶液的乳光随之降低。糖原的氮含量为0.9%，这一数值较高。
