绦虫扩展莫尼茨绦虫中蜕皮甾体的存在情况。

The occurrence of ecdysteroids in the cestode, Moniezia expansa.

作者信息

Mendis A H, Rees H H, Goodwin T W

出版信息

Mol Biochem Parasitol. 1984 Feb;10(2):123-38. doi: 10.1016/0166-6851(84)90001-x.

Abstract

The occurrence of free ecdysteroids in the sheep cestode, Moniezia expansa, was demonstrated. Significant amounts of conjugated ecdysteroids were not detected. Characterization of the free hormones by high-performance liquid chromatography monitoring fractions by radioimmunoassay, and by gas chromatography/mass spectrometry (selected ion monitoring) indicated the presence of ecdysone, 20-hydroxyecdysone and 20,26-dihydroxyecdysone. Analysis of the ecdysteroids by radioimmunoassay in segments along part of the strobila indicated that the anterior parts contained the greatest amount of hormone. GC/MS (SIM) analysis of the hormones in a strobilar segment containing the most mature proglottids suggested the presence of several ecdysteroid metabolites.

摘要

已证实绵羊绦虫扩展莫尼茨绦虫中存在游离蜕皮甾体。未检测到大量的结合蜕皮甾体。通过高效液相色谱法结合放射免疫分析法监测馏分以及通过气相色谱/质谱法（选择离子监测）对游离激素进行表征，结果表明存在蜕皮酮、20-羟基蜕皮酮和20,26-二羟基蜕皮酮。通过放射免疫分析法对虫体节片沿虫链部分进行的蜕皮甾体分析表明，前部含有的激素量最大。对含有最成熟孕节的虫链节片进行的激素气相色谱/质谱法（选择离子监测）分析表明存在几种蜕皮甾体代谢物。