Ashok A, Rao D S, Raghuramulu N
Department of Endocrinology and Metabolism, National Institute of Nutrition, Hyderabad, India.
J Nutr Sci Vitaminol (Tokyo). 1998 Apr;44(2):195-205. doi: 10.3177/jnsv.44.195.
This investigation was directed towards finding the need of vitamin D for fish. The freshwater column feeder fish Labeo rohita (Rora) was used for the study. Early fry stage fish were divided into four experimental groups of 350 each: two groups were kept in natural light, while the other two were maintained under total darkness. One each of the light and dark-grown groups was supplied dietary vitamin D3 [1,650 i.u/kg diet], whereas the other groups were fed a vitamin D-deficient diet for six months. The known vitamin D-related functions and growth parameters were studied in these four experimental groups of fish. The results showed that fish reared on vitamin D-deficient diet and in dark did not have even traces of liver vitamin D, indicating a state of vitamin D deficiency in these fish. No significant differences were observed in percent bone to body weight or dry matter of the vitamin D-deficient/supplemented groups of fish grown in light/dark. Further, it was also observed that there were no significant changes in bone and carcass ash or calcium and phosphorus content in response to vitamin D3 supplementation as compared to the groups which did not receive vitamin D3 (grown both in light and dark). Also, there was little change in several other parameters like carcass protein and lipid, mortality rates, hepatosomatic index, and feed efficiency between the vitamin D-deficient/supplemented groups of fish. Thus, these findings suggest that vitamin D may not be an essential nutrient for Rora (Labeo rohita) as a representative of freshwater fish.
本研究旨在探寻鱼类对维生素D的需求。研究使用了淡水柱食性鱼类露斯塔野鲮（Rora）。早期鱼苗阶段的鱼被分为四个实验组，每组350尾：两组置于自然光下，另外两组置于完全黑暗中。光照组和黑暗组各有一组投喂含维生素D3的饲料[1,650国际单位/千克饲料]，而其他组则投喂缺乏维生素D的饲料，为期六个月。对这四个实验组的鱼进行了已知的与维生素D相关的功能和生长参数研究。结果表明，在黑暗中饲养且投喂缺乏维生素D饲料的鱼，肝脏中甚至没有维生素D的痕迹，表明这些鱼处于维生素D缺乏状态。在光照/黑暗条件下生长的维生素D缺乏/补充组的鱼，其骨重占体重百分比或干物质方面未观察到显著差异。此外，还观察到，与未接受维生素D3的组（在光照和黑暗条件下生长）相比，补充维生素D3后，骨和胴体灰分或钙和磷含量没有显著变化。而且，在维生素D缺乏/补充组的鱼之间，胴体蛋白质和脂质、死亡率、肝体指数和饲料效率等其他几个参数也几乎没有变化。因此，这些发现表明，作为淡水鱼的代表，维生素D可能不是露斯塔野鲮（Labeo rohita）的必需营养素。
