Chen T, Zhang M Q
Department of Computer Science, State University of New York, Stony Brook 11794-4400, USA.
Yeast. 1998 Jun 15;14(8):701-10. doi: 10.1002/(SICI)1097-0061(19980615)14:8<701::AID-YEA247>3.0.CO;2-#.
A special program developed by the authors, called Pombe, identifies protein coding regions in the Schizosaccharomyces pombe genome. Linear discriminant analysis was applied to predict 5'-terminal, internal, 3'-terminal exons (coding-exon) and introns. The accuracy of the prediction was tested by cross verifications. The sensitivity, specificity and correlation coefficient for the internal exon prediction were 98.5%, 99.9% and 98.3% respectively at the nucleotide level. Open reading frames were studied and used to predict intron-less genes: 99.0% of such genes were identified with correct stopping sites. The gene structure was determined by dynamic programming and the prediction achieved 97.0% correlation coefficient at the nucleotide level. The program is available at http:(/)/clio.cshl.org/genefinder.
作者开发的一个名为Pombe的特殊程序可识别粟酒裂殖酵母基因组中的蛋白质编码区域。应用线性判别分析来预测5'端、内部、3'端外显子（编码外显子）和内含子。通过交叉验证来测试预测的准确性。在核苷酸水平上，内部外显子预测的敏感性、特异性和相关系数分别为98.5%、99.9%和98.3%。对开放阅读框进行了研究并用于预测无内含子基因：99.0%的此类基因被识别出具有正确的终止位点。通过动态规划确定基因结构，在核苷酸水平上预测的相关系数达到97.0%。该程序可在http:(/)/clio.cshl.org/genefinder获取。
