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利用傅里叶变换红外光谱法鉴别蜡样芽孢杆菌、蕈状芽孢杆菌、苏云金芽孢杆菌以及芽孢杆菌属的其他一些菌种。

Discrimination among Bacillus cereus, B. mycoides and B. thuringiensis and some other species of the genus Bacillus by Fourier transform infrared spectroscopy.

作者信息

Beattie S H, Holt C, Hirst D, Williams A G

机构信息

Hannah Research Institute, Scotland, UK.

出版信息

FEMS Microbiol Lett. 1998 Jul 1;164(1):201-6. doi: 10.1111/j.1574-6968.1998.tb13086.x.

DOI:10.1111/j.1574-6968.1998.tb13086.x
PMID:9675865
Abstract

Fourier transform infrared spectroscopy (FTIR) in conjunction with canonical variate analysis was found to be effective in discriminating among spectra of 9 representative strains of Bacillus spp., including B. cereus, B. mycoides and B. thuringiensis. The method was also able to discriminate according to species among spectra of 14 other non-type strains of B. cereus, 12 of B. mycoides and 12 of B. thuringiensis with a success rate of > 95%, even without using a prior classification of the groups by species. FTIR spectroscopy can be used for the rapid and accurate differentiation of species in the genus Bacillus that are of importance to the food and dairy industry.

摘要

傅里叶变换红外光谱法(FTIR)结合典型变量分析被发现可有效区分9种代表性芽孢杆菌属菌株的光谱,包括蜡样芽孢杆菌、蕈状芽孢杆菌和苏云金芽孢杆菌。该方法还能够在14株其他蜡样芽孢杆菌非模式菌株、12株蕈状芽孢杆菌和12株苏云金芽孢杆菌的光谱中按种类进行区分,成功率>95%,即使不事先按种类对这些菌群进行分类。傅里叶变换红外光谱法可用于快速、准确地区分芽孢杆菌属中对食品和乳制品行业具有重要意义的菌种。

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