Kamau L, Hawley W A, Lehmann T, Orago A S, Cornel A, Ke Z, Collins F H
Kenya Medical Research Institute, Nairobi, Kenya.
Heredity (Edinb). 1998 Jun;80 ( Pt 6):675-82. doi: 10.1046/j.1365-2540.1998.00327.x.
Anopheles gambiae and An. arabiensis were analysed at 30 short tandem repeat (STR) loci originally developed for use in An. gambiae. All specimens were collected from the same village in Kilifi district, coastal Kenya. All 30 loci were amplified in the An. gambiae specimens, whereas 25 out of 30 loci (83.3%) were successfully amplified in the An. arabiensis specimens. Both species had similar levels of polymorphism for the loci that were amplified (93.3% for An. gambiae and 92% for An. arabiensis). Median FST and RST values between the two species were 0.249 and 0.197, respectively, corresponding to Nm values of 0.75 and 0.51, respectively, and suggesting limited interchange of genes between these species. These, together with the relatively high Nei unbiased genetic distance (0.202) between the two sibling species, are consistent with the occurrence of sympatric species with limited gene flow. FST/RST values for individual loci varied greatly (FST range 0.00-0.87; RST range 0.00-0.73), indicating that the loci differ in their ability to measure levels of differentiation between these two species. Location of loci within paracentric inversions seems to be an important factor affecting levels of differentiation measured by the different loci.
对冈比亚按蚊和阿拉伯按蚊进行了分析，分析位点为最初开发用于冈比亚按蚊的30个短串联重复序列（STR）位点。所有样本均采集自肯尼亚沿海基利菲区的同一个村庄。在冈比亚按蚊样本中，所有30个位点均被成功扩增，而在阿拉伯按蚊样本中，30个位点中有25个（83.3%）被成功扩增。对于扩增出的位点，两个物种具有相似的多态性水平（冈比亚按蚊为93.3%，阿拉伯按蚊为92%）。两个物种之间的FST中位数和RST中位数分别为0.249和0.197，分别对应Nm值为0.75和0.51，这表明这两个物种之间的基因交换有限。这些结果，再加上这两个姐妹物种之间相对较高的Nei无偏遗传距离（0.202），与基因流有限的同域物种的出现情况一致。各个位点的FST/RST值差异很大（FST范围为0.00 - 0.87；RST范围为0.00 - 0.73），这表明不同位点在测量这两个物种之间的分化水平时能力有所不同。位于臂内倒位内的位点位置似乎是影响不同位点所测量的分化水平的一个重要因素。
