Lograno M D, Bisaccia F, Ostuni A, Daniele E, Tamburro A M
Department of Chemistry, University of Basilicata, Potenza, Italy.
Int J Biochem Cell Biol. 1998 Apr;30(4):497-503. doi: 10.1016/s1357-2725(98)00008-9.
Elastin peptides obtained in vivo from the enzymatic degradation of elastic fibers are present in the circulating human blood. In order to verify the role that these peptides may have in the regulation of the vascular tone, the activity of several peptides identified in the elastolytic digest of human elastin and some of their structural homologues has been tested. Three of these peptides show a vasorelaxant activity in isolated rat aorta precontracted by phenylephrine. The activity observed is higher in the absence of the endothelium; in these conditions the IC50 for the peptides Val-Gly-Val-Ala-Pro-Gly, Val-Gly-Val-Pro-Gly and Val-Gly-Val-Hyp-Gly was 40 +/- 2, 73 +/- 2 and 10 +/- 1 ng/ml, respectively. They are active in the range of the pathological circulating concentration and their role could be important in the regulation of vascular tone during several elastin degradative diseases.
从弹性纤维的酶促降解中在体内获得的弹性蛋白肽存在于人体循环血液中。为了验证这些肽在调节血管张力中可能发挥的作用，已对在人弹性蛋白的弹性蛋白酶消化物中鉴定出的几种肽及其一些结构同源物的活性进行了测试。其中三种肽在由去氧肾上腺素预收缩的离体大鼠主动脉中显示出血管舒张活性。在没有内皮的情况下观察到的活性更高；在这些条件下，肽Val-Gly-Val-Ala-Pro-Gly、Val-Gly-Val-Pro-Gly和Val-Gly-Val-Hyp-Gly的IC50分别为40±2、73±2和10±1 ng/ml。它们在病理循环浓度范围内具有活性，并且它们的作用在几种弹性蛋白降解性疾病期间调节血管张力方面可能很重要。
