Armone S, Voce S, dal Pozzo C, Severini G, Montanari F, Monti G, Tavolazzi L
Department of Urology, S. Maria delle Croci Hospital, Ravenna, Italy.
Arch Esp Urol. 1998 Jun;51(5):507-10.
The aim of the study is to investigate the relationship between urinary cytology, traditional indexes of malignancy of bladder carcinoma and DNA-ploidy (diploid, near-diploid, aneuploid).
52 specimens of bladder cancers (transitional cell carcinoma) were obtained from 46 patients at TURB and from 6 patients at radical cystectomy. In every specimen the nuclear content of DNA was investigated using the cytofluorimetric method. Cells were processed in standard fashion for flow cytometry with propidium iodide staining; cellular suspension was obtained mechanically. Peripheral blood lymphocytes were used as diploid standard. Bladder cytology was performed in every patient.
A relationship between DNA-ploidy and histological malignancy was observed. The largest portion of near-diploid neoplasm was observed in stage T1, grade G2 malignancies. In some cases negative bladder cytology occurred together with aneuploid DNA content, whilst in other cases positive bladder cytology occurred together with diploid DNA content. In consequence there is not always a relationship between bladder cytology and DNA-ploidy.
Since observed correlations may point out some neoplasms with particular biological behaviour, follow-up of near-diploid malignancies is of great interest.
本研究旨在探讨尿细胞学检查、膀胱癌传统恶性指标与DNA倍体（二倍体、近二倍体、非整倍体）之间的关系。
从46例行经尿道膀胱肿瘤切除术（TURB）的患者及6例行根治性膀胱切除术的患者中获取52份膀胱癌（移行细胞癌）标本。采用细胞荧光分析法检测每份标本的DNA核含量。细胞经碘化丙啶染色后按标准流程进行流式细胞术检测；通过机械方法获得细胞悬液。以外周血淋巴细胞作为二倍体标准。对每位患者进行膀胱细胞学检查。
观察到DNA倍体与组织学恶性程度之间存在关联。在T1期、G2级恶性肿瘤中观察到近二倍体肿瘤的比例最高。在某些情况下，膀胱细胞学检查结果为阴性，但DNA含量为非整倍体；而在其他情况下，膀胱细胞学检查结果为阳性，但DNA含量为二倍体。因此，膀胱细胞学检查与DNA倍体之间并不总是存在关联。
由于观察到的相关性可能指出某些具有特定生物学行为的肿瘤，因此对近二倍体恶性肿瘤的随访具有重要意义。
