Durand S, Lightner D V, Bonami J R
UMR 219 CNRS/IFREMER, UMII, Montpellier, France.
Dis Aquat Organ. 1998 Apr 3;32(3):237-9. doi: 10.3354/dao032237.
BP-type viruses infect wild and farmed shrimp species in the Americas and can cause high mortality in larval stages. Histologically, BP disease is characterized by the presence of occlusion bodies (OBs) in the nucleus of hepatopancreatic epithelial cells. By in situ hybridization using different molecular probes, we tested shrimp infected with BP-type viruses collected from several geographical areas. In one shrimp, a case of double infection by 2 different BP-type viruses was noted. This has not been previously reported. The molecular probes recognized only the Pacific strain of BP. This specificity of the probe was confirmed by in situ hybridization tests with some infected shrimp collected from the Atlantic and Pacific coasts. The probes reacted only with infected shrimp obtained from the Pacific coast. These results suggest the existence of at least 2 different BP-type viruses and show that specific probes can be used to differentiate between them.
BP型病毒感染美洲的野生和养殖虾类物种，并可在幼体阶段导致高死亡率。组织学上，BP病的特征是肝胰腺上皮细胞核中存在包涵体（OBs）。通过使用不同分子探针的原位杂交，我们检测了从几个地理区域收集的感染BP型病毒的虾。在一只虾中，发现了由2种不同BP型病毒双重感染的病例。此前尚未有过此类报道。分子探针仅识别BP的太平洋毒株。通过对从大西洋和太平洋沿岸收集的一些受感染虾进行原位杂交试验，证实了探针的这种特异性。这些探针仅与从太平洋沿岸获得的受感染虾发生反应。这些结果表明至少存在2种不同的BP型病毒，并表明可以使用特异性探针来区分它们。
