Choi Y S, Kim J Y, Wee W R, Lee J H
Department of Ophthalmology, College of Medicine, Chungbuk National University, Cheongju, South Korea.
Cornea. 1998 Jul;17(4):389-95. doi: 10.1097/00003226-199807000-00009.
We investigated the influence of amniotic membrane application on corneal wound healing after excimer laser photorefractive keratectomy (PRK) in rabbits.
PRK of -9.9 D with an optical zone of 5.0 mm was performed on each right eye of 34 pigmented rabbits, which had been divided into two groups. In 17 eyes, preserved human amniotic membrane was applied in such a way that it covered the entire cornea for 48 h (amniotic group), and the other eyes formed the control group. The area of epithelial defect, inflammatory cell infiltration, the number of anterior stromal keratocytes, and corneal haze were evaluated.
In all animals, the epithelium had healed completely within 3 days, and there was no difference between the two groups. At postoperative 12 and 24 h, the numbers of stromal inflammatory cells in the amniotic group were significantly lower than those in the control group (p = 0.009), and the numbers of anterior stromal keratocytes were significantly higher (p < 0.05). At postoperative weeks 2, 4, 6, 8, and 12, corneal haze scores in the amniotic group were lower than those in the control group (p < 0.05), and at postoperative week 12, the number of anterior stromal keratocytes in the amniotic group was significantly lower than that in the control group (p = 0.002).
The application of amniotic membrane after PRK reduces keratocyte proliferation and corneal haze during corneal wound healing, possibly by reducing the infiltration of inflammatory cells and loss of keratocytes in the ablation area during the early postoperative period.
我们研究了羊膜应用对兔准分子激光屈光性角膜切削术（PRK）后角膜伤口愈合的影响。
对34只色素兔的右眼进行-9.9 D、光学区为5.0 mm的PRK，将其分为两组。17只眼应用保存的人羊膜，使其覆盖整个角膜48小时（羊膜组），其余眼作为对照组。评估上皮缺损面积、炎性细胞浸润、前基质角膜细胞数量和角膜混浊情况。
所有动物的上皮在3天内完全愈合，两组之间无差异。术后12小时和24小时，羊膜组的基质炎性细胞数量明显低于对照组（p = 0.009），前基质角膜细胞数量明显更高（p < 0.05）。术后第2、4、6、8和12周，羊膜组的角膜混浊评分低于对照组（p < 0.05），术后第12周，羊膜组的前基质角膜细胞数量明显低于对照组（p = 0.002）。
PRK术后应用羊膜可减少角膜伤口愈合过程中的角膜细胞增殖和角膜混浊，可能是通过减少术后早期消融区域的炎性细胞浸润和角膜细胞丢失来实现的。
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