Woo H M, Kim M S, Kweon O K, Kim D Y, Nam T C, Kim J H
College of Veterinary Medicine, Seoul National University, Suwon, Korea.
Br J Ophthalmol. 2001 Mar;85(3):345-9. doi: 10.1136/bjo.85.3.345.
To investigate if the amniotic membrane (AM) promotes epithelial migration while inhibiting stromal remodelling associated with corneal haze after excimer laser keratectomy.
A wound 150 microm in depth and 6.0 mm in diameter was produced in 40 rabbits using an excimer laser. One eye was randomly chosen to be covered by the AM while the other eye served as a control. Epithelial wound healing was evaluated, together with any morphological changes of the anterior stroma connected with corneal haze. These morphological changes were histopathologically analysed using dichlortriazinyl aminofluorescein (DTAF), Masson trichrome staining, and an image analyser.
The AM group had a short latent phase followed by fast epithelial healing (p<0.001) during the early wound healing period and a significant decrease in the inflammatory response, together with a smaller change in the number of keratocytes than the control group. The mean thickness of the regenerated stroma was significantly thinner in the AM group than in the control group at 8 weeks (p<0.0001). The AM group had a more regular architecture of regenerated stromal lamella at 8 weeks and significantly less haze after 4 weeks than the control group (p<0.05).
Use of the AM as a dressing on a corneal wound created by excimer laser surgery, in which severe haze is expected, may induce rapid epithelial healing with less inflammatory response. The AM may inhibit the irregular synthesis of stromal collagen that is associated with corneal haze.
研究准分子激光角膜切削术后羊膜（AM）是否能促进上皮细胞迁移，同时抑制与角膜混浊相关的基质重塑。
使用准分子激光在40只兔子身上制作一个深度为150微米、直径为6.0毫米的伤口。随机选择一只眼睛用羊膜覆盖，另一只眼睛作为对照。评估上皮伤口愈合情况以及与角膜混浊相关的前基质的任何形态变化。使用二氯三嗪基氨基荧光素（DTAF）、Masson三色染色和图像分析仪对这些形态变化进行组织病理学分析。
在伤口愈合早期，羊膜组的潜伏期较短，随后上皮愈合迅速（p<0.001），炎症反应明显减轻，与对照组相比，角膜细胞数量变化较小。8周时，羊膜组再生基质的平均厚度明显薄于对照组（p<0.0001）。8周时，羊膜组再生基质板层结构更规则，4周后混浊明显少于对照组（p<0.05）。
在预期会出现严重混浊的准分子激光手术造成的角膜伤口上使用羊膜作为敷料，可能会诱导上皮快速愈合，炎症反应较轻。羊膜可能会抑制与角膜混浊相关的基质胶原的不规则合成。
