神经酰胺从内质网到高尔基体的转运不是由囊泡介导的。
Ceramide transport from endoplasmic reticulum to Golgi apparatus is not vesicle-mediated.
作者信息
Kok J W, Babia T, Klappe K, Egea G, Hoekstra D
机构信息
University of Groningen, Department of Physiological Chemistry, A. Deusinglaan 1, 9713 AV Groningen, The Netherlands.
出版信息
Biochem J. 1998 Aug 1;333 ( Pt 3)(Pt 3):779-86. doi: 10.1042/bj3330779.
Abstract
Ceramide (Cer) transfer from the endoplasmic reticulum (ER) to the Golgi apparatus was measured under conditions that block vesicle-mediated protein transfer. This was done either in intact cells by reducing the incubation temperature to 15 degreesC, or in streptolysin O-permeabilized cells by manipulating the intracellular environment. In both cases, Cer transfer was not inhibited, as demonstrated by the biosynthesis of ceramide monohexosides and sphingomyelin (SM) de novo from metabolically (with [14C]serine) labelled Cer. This assay is based on the knowledge that Cer is synthesized, starting from serine and palmitoyl-CoA, at the ER, whereas glycosphingolipids and SM are synthesized in the (early) Golgi apparatus. Formation of [14C]glycosphingolipids and [14C]SM was observed under conditions that block vesicle-mediated vesicular stomatitis virus glycoprotein transport. These results indicate that [14C]Cer is transferred from ER to Golgi by a non-vesicular mechanism.
摘要
在内质网（ER）到高尔基体的神经酰胺（Cer）转运是在阻断囊泡介导的蛋白质转运的条件下进行测量的。这通过两种方式实现，一是在完整细胞中将孵育温度降至15℃，二是在经链球菌溶血素O通透处理的细胞中通过操纵细胞内环境来实现。在这两种情况下，神经酰胺转运均未受到抑制，从代谢性（用[14C]丝氨酸）标记的神经酰胺从头合成神经酰胺单己糖苷和鞘磷脂（SM）即可证明这一点。该检测基于这样的知识，即神经酰胺从丝氨酸和棕榈酰辅酶A开始在ER合成，而糖鞘脂和SM在（早期）高尔基体中合成。在阻断囊泡介导的水疱性口炎病毒糖蛋白转运的条件下观察到了[14C]糖鞘脂和[14C]SM的形成。这些结果表明，[14C]神经酰胺通过非囊泡机制从内质网转移到高尔基体。
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本文引用的文献
1
A rapid method of total lipid extraction and purification.一种快速的总脂质提取与纯化方法。
Can J Biochem Physiol. 1959 Aug;37(8):911-7. doi: 10.1139/o59-099.
2
Role of NAD+ and ADP-ribosylation in the maintenance of the Golgi structure.NAD⁺与ADP核糖基化在高尔基体结构维持中的作用。
J Cell Biol. 1997 Dec 1;139(5):1109-18. doi: 10.1083/jcb.139.5.1109.
3
Characterization of ceramide synthesis. A dihydroceramide desaturase introduces the 4,5-trans-double bond of sphingosine at the level of dihydroceramide.神经酰胺合成的特征。二氢神经酰胺去饱和酶在二氢神经酰胺水平引入鞘氨醇的4,5-反式双键。
J Biol Chem. 1997 Sep 5;272(36):22432-7. doi: 10.1074/jbc.272.36.22432.
4
Dihydroceramide biology. Structure-specific metabolism and intracellular localization.二氢神经酰胺生物学。结构特异性代谢与细胞内定位。
J Biol Chem. 1997 Aug 22;272(34):21128-36. doi: 10.1074/jbc.272.34.21128.
5
Transport of sphingomyelin to the cell surface is inhibited by brefeldin A and in mitosis, where C6-NBD-sphingomyelin is translocated across the plasma membrane by a multidrug transporter activity.鞘磷脂向细胞表面的转运受到布雷菲德菌素A的抑制，并且在有丝分裂过程中，C6-NBD-鞘磷脂通过一种多药转运蛋白活性跨质膜转运。
J Cell Sci. 1997 Jan;110 ( Pt 1):75-83. doi: 10.1242/jcs.110.1.75.
6
Protein trafficking along the exocytotic pathway.蛋白质沿胞吐途径的运输。
Bioessays. 1993 Apr;15(4):231-8. doi: 10.1002/bies.950150403.
7
The long-chain sphingoid base of sphingolipids is acylated at the cytosolic surface of the endoplasmic reticulum in rat liver.在大鼠肝脏中，鞘脂的长链鞘氨醇碱在内质网的胞质表面被酰化。
Biochem J. 1993 Mar 15;290 ( Pt 3)(Pt 3):751-7. doi: 10.1042/bj2900751.
8
Ceramide excluded from cell-free vesicular lipid transfer from endoplasmic reticulum to Golgi apparatus. Evidence for lipid sorting.神经酰胺被排除在从内质网到高尔基体的无细胞囊泡脂质转运之外。脂质分选的证据。
Biochim Biophys Acta. 1993 Feb 23;1146(1):9-16. doi: 10.1016/0005-2736(93)90332-t.
9
Differential metabolism and trafficking of sphingolipids in differentiated versus undifferentiated HT29 cells.分化与未分化的HT29细胞中鞘脂的差异代谢与转运
Int J Cancer. 1993 Jul 9;54(5):839-45. doi: 10.1002/ijc.2910540519.
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