Ries J C, Schreiner D, Steininger H, Girod S C
Department of Oral and Maxillofacial Surgery, University of Erlangen, Germany.
Anticancer Res. 1998 May-Jun;18(3B):2031-6.
In many studies the detection of p53 protein by immunohistochemistry (IHC) with one antibody is assumed to be consistent with a mutation of the gene. To determine the correlation of protein detection by immunohistochemistry and gene mutation, paraffin embedded specimens of 60 oral leukoplakias (OL) and 73 oral squamous cell carcinomas (OSCC) were analysed by IHC with two different antibodies (Do7; Pab 1801), PCR-SSCP and sequencing. In 98% of OLs and 94% of OSSCs p53 protein expression was detected with at least one antibody. Only 49% of all tissue specimen were positive with both antibodies. Molecular analysis of the same specimen showed mutations of the p53 gene in 13.3% of OLs and 9, 6% of OSCCs. p53 protein expression was not detected by IHC in 3 out of 7 OSCC with p53 mutations. According to these results detection of p53 expression by IHC is not always correlated with p53 gene mutation and failure to detect p53 protein by IHC does not prove the absence of mutation of the gene in the carcinogenesis in the oral mucosa.
在许多研究中，用一种抗体通过免疫组织化学（IHC）检测p53蛋白被认为与该基因的突变一致。为了确定免疫组织化学检测蛋白与基因突变之间的相关性，我们用两种不同的抗体（Do7；Pab 1801）、PCR-SSCP和测序对60例口腔白斑（OL）和73例口腔鳞状细胞癌（OSCC）的石蜡包埋标本进行了分析。在98%的OL和94%的OSCC中，至少用一种抗体检测到了p53蛋白表达。所有组织标本中只有49%两种抗体均呈阳性。对相同标本的分子分析显示，13.3%的OL和9.6%的OSCC存在p53基因突变。在7例有p53突变的OSCC中，有3例通过免疫组织化学未检测到p53蛋白表达。根据这些结果，免疫组织化学检测p53表达并不总是与p53基因突变相关，免疫组织化学未能检测到p53蛋白也不能证明口腔黏膜癌变中该基因不存在突变。
