Kaur Harpreet, Hazarey Vinay, Sharma Gitika, Gosavi Suchitra, Pal Rana Agk, Gupta Vandana
Division of Oral Pathology and Microbiology, Centre for Dental Education and Research, All India Institute of Medical Sciences, Delhi, India.
Datta Meghe Institute of Medical Sciences Deemed University, Wardha, India.
Indian J Otolaryngol Head Neck Surg. 2024 Feb;76(1):103-111. doi: 10.1007/s12070-023-04092-7. Epub 2023 Jul 26.
The purpose of this study was to examine the immunohistochemical expression of p53 and cytokeratin 19 (CK19) in normal oral mucosa (NOM) and oral squamous cell carcinoma (OSCC) and their association with histopathological differentiation grade. The secondary goal was to see if there was any correlation between p53 and CK19 expression in NOM and OSCC. A hospital-based retrospective analysis was conducted in which 40 NOM and 45 OSCC samples were acquired from archives and stained with mouse monoclonal antibodies p53 and CK19. For both the NOM and OSCC groups, the proportion of positively stained cells, staining intensity, and staining index were calculated. p53 immunoexpression revealed that 85% of positively stained cells in the NOM basal layer had a low staining index (mean ± SD 1.87 ± 0.34), whereas 66.7% of positively stained cells in the OSCC had a high staining index (mean ± SD 5.63 ± 3.02). When NOM and OSCC were compared, there was a statistically significant difference in staining intensity. However, despite a linear increase in the percentage of positive cells from well to poorly differentiated, the comparison between histopathological grades was non-significant. CK19 exhibited 18.5% positively stained cells in the NOM basal layer with a low staining index (mean ± SD 1.57 ± 0.53), whereas OSCC samples showed 4.44% immunopositivity with a high staining index. p53 is a marker of oral carcinogenesis independent of histological grade and CK19 expression. Further, CK19 is a marker of dysfunctional epithelial differentiation but lacks sensitivity and specificity; however, it demands further multicentric studies with a large sample size to draw definitive conclusions.
The online version contains supplementary material available at 10.1007/s12070-023-04092-7.
本研究的目的是检测p53和细胞角蛋白19（CK19）在正常口腔黏膜（NOM）和口腔鳞状细胞癌（OSCC）中的免疫组化表达及其与组织病理学分化程度的关系。次要目标是观察NOM和OSCC中p53与CK19表达之间是否存在相关性。进行了一项基于医院的回顾性分析，从档案中获取40份NOM样本和45份OSCC样本，并用小鼠单克隆抗体p53和CK19进行染色。计算了NOM组和OSCC组中阳性染色细胞的比例、染色强度和染色指数。p53免疫表达显示，NOM基底层85%的阳性染色细胞染色指数较低（平均值±标准差1.87±0.34），而OSCC中66.7%的阳性染色细胞染色指数较高（平均值±标准差5.63±3.02）。比较NOM和OSCC时，染色强度存在统计学显著差异。然而，尽管从高分化到低分化阳性细胞百分比呈线性增加，但组织病理学分级之间的比较无统计学意义。CK19在NOM基底层显示18.5%的阳性染色细胞，染色指数较低（平均值±标准差1.57±0.53），而OSCC样本显示4.44%的免疫阳性，染色指数较高。p53是口腔癌发生的标志物，与组织学分级和CK19表达无关。此外，CK19是上皮分化功能障碍的标志物，但缺乏敏感性和特异性；然而，需要进一步开展多中心、大样本量研究以得出明确结论。
在线版本包含可在10.1007/s12070-023-04092-7获取的补充材料。
