Scheifele C, Schlechte H, Bethke G, Reichart P A
Abteilung für Oralchirurgie und zahnärztliche Röntgenologie, Zentrum für Zahnmedizin, Universitätsklinikum Charité der Humboldt-Universität, Augustenburger Platz 1, 13353 Berlin,
Mund Kiefer Gesichtschir. 2002 Nov;6(6):410-4. doi: 10.1007/s10006-002-0425-0. Epub 2002 Sep 13.
The aim of this study was to determine the prevalence of mutations of the tumour suppressor gene TP53 in oral leukoplakias.
Brush biopsy specimens of 43 oral leukoplakias, 26 oral squamous cell carcinomas (OSCC) for reference, and the oral mucosa of 4 clinically normal volunteers were collected. DNA of the critical exons 5-8 was analysed by temperature gradient gel electrophoresis (TGGE).
The prevalence of mutations was 57.7% in OSCC, 39.5% in leukoplakias and 0% in controls. The highest frequency of mutations was found in exon 5 (46.2%) in OSCC and in exon 6 (23.3%) in leukoplakia. More than one mutation was detected in 26.9% of OSCC and 7% of leukoplakia specimens. At least one mutation was found in 37.5% of T1 OSCC and 100% of T4 OSCC specimens and in 37.1% of the L1 leukoplakia and 100% of L3 leukoplakia specimens.
TP53 mutations could be a useful prognostic indicator in precancerous oral lesions. Although the brush biopsy technique appears simple clinically, further investigations are necessary to specify the implications of genetic analysis.
本研究旨在确定口腔白斑中肿瘤抑制基因TP53的突变发生率。
收集43例口腔白斑、26例作为对照的口腔鳞状细胞癌（OSCC）的刷取活检标本以及4名临床正常志愿者的口腔黏膜。采用温度梯度凝胶电泳（TGGE）分析关键外显子5 - 8的DNA。
OSCC的突变发生率为57.7%，白斑为39.5%，对照组为0%。OSCC中突变频率最高的是外显子5（46.2%），白斑中是外显子6（23.3%）。26.9%的OSCC标本和7%的白斑标本检测到不止一处突变。在37.5%的T1期OSCC标本、100%的T4期OSCC标本、37.1%的L1期白斑标本和100%的L3期白斑标本中至少发现一处突变。
TP53突变可能是口腔癌前病变有用的预后指标。尽管刷取活检技术在临床上看似简单，但仍需进一步研究以明确基因分析的意义。
