Yokoyama T, Oda M, Seino Y
Department of Pediatrics, Okayama University Medical School, Japan.
Pediatr Allergy Immunol. 1998 May;9(2):91-6.
The inflammatory process in aseptic meningitis is generally mild and self-limited. To evaluate control mechanisms underlying this process, we determined interleukin (IL)-1 beta and IL-1 receptor antagonist (IL-1ra) concentrations, using enzyme linked immunosorbent assay, in cerebrospinal fluid from patients with aseptic meningitis (n = 55, median age [range]; 5.0 [1-15] years). We cross-sectionally analyzed the relationship of IL-1 beta and IL-1ra levels to leucocyte count in terms of interval between clinical onset and lumbar puncture. IL-1 beta levels were highest in the cerebrospinal fluid obtained between 12 and 24 hours after onset (n = 23, median: 2.30 pg/ml). The IL-1 beta level was significantly correlated with leucocyte count. On the other hand, IL-1ra level tended to increase over a longer interval, reaching a plateau between 24 and 36 hours after onset (n = 13, median: 5190 pg/ml). The molar ratio of IL-1ra to IL-1 beta was thus lowest between 12 and 24 hours after onset (median: 2341), which coincided with the peak increase in leucocyte count. The results are compatible with the idea that IL-1ra should be important for the regulation of IL-1 beta activity associated with leucocyte migration in aseptic meningitis.
无菌性脑膜炎中的炎症过程通常较为轻微且具有自限性。为评估该过程潜在的调控机制，我们采用酶联免疫吸附测定法，测定了无菌性脑膜炎患者（n = 55，中位年龄[范围]；5.0[1 - 15]岁）脑脊液中的白细胞介素（IL）-1β和IL-1受体拮抗剂（IL-1ra）浓度。我们根据临床发病与腰椎穿刺之间的时间间隔，对IL-1β和IL-1ra水平与白细胞计数之间的关系进行了横断面分析。发病后12至24小时获取的脑脊液中IL-1β水平最高（n = 23，中位值：2.30 pg/ml）。IL-1β水平与白细胞计数显著相关。另一方面，IL-1ra水平在较长时间间隔内呈上升趋势，在发病后24至36小时达到平台期（n = 13，中位值：5190 pg/ml）。因此，发病后12至24小时IL-1ra与IL-1β的摩尔比最低（中位值：2341），这与白细胞计数的峰值增加相吻合。这些结果与以下观点相符，即IL-ra对于无菌性脑膜炎中与白细胞迁移相关的IL-1β活性的调节应具有重要作用。
