大鼠血清降钙素的放射免疫测定法。

A radioimmunoassay for serum calcitonin in the rat.

作者信息

Bell N H, Queener S F

出版信息

Proc Soc Exp Biol Med. 1976 Sep;152(4):516-9. doi: 10.3181/00379727-152-39430.

Abstract

A radioimmunoassay for serum calcitonin in the rat is described. Materials for assay of human calcitonin are employed. The sensitivity of the assay is 3 pg and corresponds to a lower limit of detection of 60 pg/ml for peripheral serum. The normal range is up to 650 pg/ml in rats weighing between 125 and 200 g. Serum calcitonin is not detectable in thyroparathyroidectomized animals. It increases and varies directly with the serum calcium after intraperitoneal administration of the ion. Values for serum calcitonin in male and female rats are comparable under basal conditions and after calcium. The radioimmunoassay should allow the further characterization of calcitonin secretion in response to physiologic and pathophysiologic stimuli in this species.

摘要

本文描述了一种用于大鼠血清降钙素的放射免疫测定法。采用了人降钙素测定的材料。该测定法的灵敏度为3皮克，外周血清的检测下限为60皮克/毫升。体重在125至200克之间的大鼠正常范围高达650皮克/毫升。在甲状腺甲状旁腺切除的动物中检测不到血清降钙素。腹腔注射离子后，血清降钙素会升高，并与血清钙直接相关。在基础条件下和补钙后，雄性和雌性大鼠的血清降钙素值相当。放射免疫测定法应有助于进一步表征该物种对生理和病理生理刺激的降钙素分泌情况。