Mortensen S H, Nilsen R K, Hjeltnes B
Institute of Marine Research, Department of Aquaculture, Bergen, Norway.
Dis Aquat Organ. 1998 May 14;33(1):67-71. doi: 10.3354/dao033067.
An infectious pancreatic necrosis virus (IPNV) belonging to the Sp serotype, isolated from scallops Pecten maximus, was propagated and suspended in sterile water and cell culture medium with different salinities and incubated at temperatures ranging from -80 to 40 degrees C. Virus stability was examined by measuring virus titers under different storage conditions. Virus titers were also measured after repeated freezing and thawing, and in incubated sterile filtered scallop hepatopancreas, haemolymph and crystalline style samples, and salmon Salmo salar kidney homogenate. The virus was stable under most storage conditions. Temperatures ranging from -80 to +20 degrees C, as well as salinities from 0 to 40/1000, did not seem to influence the stability of the virus. A reduction was observed above 20 degrees C. Each freezing and thawing procedure resulted in a reduction of the virus titer. This reduction was larger at -80 than at -20 degrees C. The IPNV persisted for a long period in sterile filtered scallop haemolymph, dissolved crystalline style and hepatopancreas. In kidney homogenate from IPNV-infected salmon the virus titers were reduced at least 10-fold during the first day of incubation at all temperatures tested. When virus was propagated in cell culture and subsequently mixed with non-infected salmon kidney homogenate, the virus proved more persistent. Our results illustrate the importance of rapid and standardised laboratory processing of potentially virus-containing tissue samples, and are relevant when considering laboratory storage of samples containing IPNV.
从大扇贝（Pecten maximus）中分离出的一株属于Sp血清型的传染性胰腺坏死病毒（IPNV），在不同盐度的无菌水和细胞培养基中进行增殖和悬浮，并在-80至40摄氏度的温度范围内孵育。通过测量不同储存条件下的病毒滴度来检测病毒稳定性。在反复冻融后，以及在无菌过滤的扇贝肝胰腺、血淋巴和晶杆样品以及鲑鱼（Salmo salar）肾脏匀浆中孵育后，也测量了病毒滴度。该病毒在大多数储存条件下都很稳定。-80至+20摄氏度的温度以及0至40/1000的盐度似乎都不会影响病毒的稳定性。在20摄氏度以上观察到病毒滴度有所下降。每次冻融过程都会导致病毒滴度降低。在-80摄氏度时这种降低比在-20摄氏度时更大。IPNV在无菌过滤的扇贝血淋巴、溶解的晶杆和肝胰腺中能长期存活。在所有测试温度下，在感染IPNV的鲑鱼肾脏匀浆中孵育的第一天，病毒滴度至少降低了10倍。当病毒在细胞培养中增殖并随后与未感染的鲑鱼肾脏匀浆混合时，该病毒表现出更强的持久性。我们的结果说明了对可能含有病毒的组织样本进行快速和标准化实验室处理的重要性，并且在考虑含有IPNV的样本的实验室储存时具有参考价值。
