全血和红细胞悬液中鸡血红蛋白的氧亲和力

The oxygen affinity of chicken haemoglobin in whole blood and erythrocyte suspensions.

作者信息

Wells R M

出版信息

Respir Physiol. 1976 Jul;27(1):24-31. doi: 10.1016/0034-5687(76)90015-3.

Abstract

The oxygen equilibrium of whole chicken blood has a P50 of 52.3 mm Hg and a Hill coefficient of 2.6 at pH 7.4 and 37 degrees C when determined with new microtechniques which are not vitiated by cellular respiration. The apparent failure of the haemoglobin to reach full saturation at arterial PO2 IS DISCUSSED IN RELATION TO THE HAEMoglobin-oxygen equilibrium concept. The low affinity observed is due to intraerythrocytic inositol pentaphosphate (IPP), and the affinity of a haemolysate "stripped" of IPP is greatly increased (deltalog P50 =1.25). Unlike the mamalian analogue 2, 3-diphosphoglycerate, IPP concentration of whole blood does not decrease after incubation for 10 hr at 37 degreesC.

摘要

当采用不受细胞呼吸影响的新微技术测定时，在pH值7.4和37摄氏度条件下，全鸡血的氧平衡的P50为52.3毫米汞柱，希尔系数为2.6。结合血红蛋白-氧平衡概念，讨论了血红蛋白在动脉血氧分压下未能达到完全饱和这一明显现象。观察到的低亲和力是由于红细胞内的肌醇五磷酸（IPP）所致，去除IPP的溶血产物的亲和力大大增加（Δlog P50 = 1.25）。与哺乳动物类似物2,3-二磷酸甘油酸不同，全血的IPP浓度在37摄氏度孵育10小时后不会降低。