Pang Y, Zeng L, Kurochkin A V, Zuiderweg E R
Biophysics Research Division, University of Michigan, Ann Arbor 48109-1055, USA.
J Biomol NMR. 1998 Feb;11(2):185-90. doi: 10.1023/a:1008229723544.
A new triple-resonance pulse sequence, 3D HNHCACO, is introduced and discussed, which identifies sequential correlations of the backbone nuclei (H alpha (i-1), C alpha (i-1), C(i-1), NH(i), N(i)) of doubly labeled proteins in H2O. The three-dimensional (3D) method utilizes a recording of 15N and 13C resonances in a single indirect time domain, the 13C' resonance in another indirect time domain, and detects both NH and H alpha protons. A bidirectional coherence transfer (NH(i) <--> N(i) <--> C(i-1) <--> C alpha (i-1) <--> H alpha (i-1)) is effectuated, resulting in a single high-resolution 3D spectrum that contains the frequencies of all five backbone nuclei. The experiment was applied to the 12.3 kDa ribonuclease from Bacillus intermedius (Binase).
本文介绍并讨论了一种新的三共振脉冲序列——3D HNHCACO，该序列可识别H2O中双标记蛋白质主链核（Hα(i - 1)、Cα(i - 1)、C(i - 1)、NH(i)、N(i)）的顺序相关性。这种三维（3D）方法在单个间接时域中记录15N和13C共振，在另一个间接时域中记录13C′共振，并同时检测NH和Hα质子。实现了双向相干转移（NH(i) <--> N(i) <--> C(i - 1) <--> Cα(i - 1) <--> Hα(i - 1)），从而得到一个包含所有五个主链核频率的单一高分辨率3D谱。该实验应用于来自中间芽孢杆菌的12.3 kDa核糖核酸酶（Binase）。
