Farmer B T, Mueller L
Macromolecular NMR, Pharmaceutical Research Institute, Bristol-Myers Squibb, Princeton, NJ 08543-4000.
J Biomol NMR. 1994 Sep;4(5):673-87. doi: 10.1007/BF00404277.
The simultaneous acquisition of a 4D gradient-enhanced and sensitivity-enhanced [13C,15N]/[15N,15N]-separated NOESY is presented for the 74-residue [13C,15N]-labeled N-terminal SH3 domain of mGrb2 complexed with a peptide fragment from mSOS-2 in 90% H2O. The method readily accommodates different 13C and 15N spectral widths, but requires that the same number of increments be collected for both 13C and 15N in the simultaneous dimension (F2). For purposes of display and analysis, the two 4D spectra can be deconvolved during the processing stage by the appropriate linear combination of separately stored FIDs. Compared to collecting each of these two 4D data sets separately, the presented method is a factor (2)1/2 more efficient in sensitivity per unit acquisition time. The interleaved nature of this method may also lead to improved peak registration between the two 4D spectra.
本文介绍了一种同时采集4D梯度增强和灵敏度增强的[13C,15N]/[15N,15N]分离NOESY的方法，用于在90% H2O中与来自mSOS-2的肽片段复合的74个残基的[13C,15N]标记的mGrb2 N端SH3结构域。该方法很容易适应不同的13C和15N光谱宽度，但要求在同时维度（F2）中为13C和15N收集相同数量的增量。为了显示和分析的目的，在处理阶段可以通过分别存储的FID的适当线性组合对两个4D光谱进行反卷积。与分别收集这两个4D数据集相比，所提出的方法在每单位采集时间的灵敏度方面提高了（2）1/2倍。该方法的交错性质也可能导致两个4D光谱之间的峰匹配得到改善。
