Goliaei B, Deizadji A
Institute of Biochemistry and Biophysics, University of Tehran, Iran.
Leuk Res. 1998 Aug;22(8):705-10. doi: 10.1016/s0145-2126(98)00061-7.
We have studied the effects of granulocyte-macrophage colony-stimulating factor (GM-CSF) and hyperthermia individually and in combination on the cell growth and differentiation of human monoblastic leukemia cell line U937. Several criteria were used to evaluate the differentiation of these cells, including the reduction in the plating efficiency and cell growth, the ability to phagocytize latex particles, the reduction of nitro blue tetrazolium (NBT), and development of surface antigenic markers. Hyperthermia alone was able to inhibit cell proliferation, reduce cell viability, and induce differentiation. In the range of 41-43 degrees C, the major effect of hyperthermia was cell differentiation induction as judged by above criteria. On average, hyperthermia induced differentiation in 32% of cells. GM-CSF was able to induce differentiation in 37% of U937 cells as judged by similar criteria. The combined treatment with GM-CSF and hyperthermia resulted in the differentiation of 60% of U937 cells. The extent of differentiation obtained is comparable or better than other combinatorial treatments using various cytokines or cytokines and chemical reagents reported before.
我们研究了粒细胞-巨噬细胞集落刺激因子（GM-CSF）和热疗单独及联合应用对人单核细胞白血病细胞系U937细胞生长和分化的影响。采用了多项标准来评估这些细胞的分化情况，包括平板接种效率和细胞生长的降低、吞噬乳胶颗粒的能力、硝基蓝四氮唑（NBT）的还原以及表面抗原标志物的出现。单独热疗能够抑制细胞增殖、降低细胞活力并诱导分化。在41-43摄氏度范围内，根据上述标准判断，热疗的主要作用是诱导细胞分化。平均而言，热疗诱导32%的细胞分化。根据类似标准判断，GM-CSF能够诱导37%的U937细胞分化。GM-CSF与热疗联合处理导致60%的U937细胞分化。所获得的分化程度与之前报道的使用各种细胞因子或细胞因子与化学试剂的其他联合处理相当或更好。
