Struhl K
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA.
Genes Funct. 1997 Feb;1(1):5-9. doi: 10.1046/j.1365-4624.1997.00004.x.
Transcription factor TFIID, a central component of the eukaryotic RNA polymerase II transcription machinery, is a multiprotein complex containing the TATA-binding protein (TBP) and TBP-associated factors (TAFs). In vitro, TAFs are required for the response to activator proteins, but they are dispensible for basal transcription. However, recent work in yeast cells indicates that TAFs are not generally required for transcriptional activation, but rather have selective effects on gene expression. Molecular mechanisms for these observations are considered.
转录因子TFIID是真核生物RNA聚合酶II转录机制的核心组成部分，是一种包含TATA结合蛋白（TBP）和TBP相关因子（TAFs）的多蛋白复合物。在体外，TAFs是激活蛋白应答所必需的，但对于基础转录来说它们是可有可无的。然而，最近在酵母细胞中的研究表明，TAFs通常不是转录激活所必需的，而是对基因表达具有选择性作用。本文考虑了这些观察结果的分子机制。
