基于fosmid的嗜热嗜酸栖热菌基因组图谱及474个基因的鉴定。
A fosmid-based genomic map and identification of 474 genes of the hyperthermophilic archaeon Pyrobaculum aerophilum.
作者信息
Fitz-Gibbon S, Choi A J, Miller J H, Stetter K O, Simon M I, Swanson R, Kim U J
机构信息
Department of Microbiology and Molecular Genetics and The Molecular Biology Institute, University of California at Los Angeles, 90095-1489, USA.
出版信息
Extremophiles. 1997 Feb;1(1):36-51. doi: 10.1007/s007920050013.
We have constructed a physical map of the approximately 1.7-Mb genome of the hyperthermophilic archaeon Pyrobaculum aerophilum. Derived from a 12x coverage genomic fosmid library with an average insert size of 36 Kb, the map consists of a single circular contig of 96 overlapping fosmid clones with 211 markers ordered along them. One hundred of the sequence markers have strong similarities to known genes. Many overlaps were also checked using restriction fingerprint analysis. This map is an important step in the elucidation of the sequence of the entire genome of Pyrobaculum aerophilum. To this end we have determined more than 95% of the genome with 15,000 random sequences. Each sequence has been screened against the public sequence databases to identify similarities to known genes. We report here a list of the 474 putative genes we have identified.
我们构建了嗜热嗜酸栖热菌（Pyrobaculum aerophilum）约1.7兆碱基基因组的物理图谱。该图谱源自一个平均插入片段大小为36千碱基的12倍覆盖基因组fosmid文库，由96个重叠的fosmid克隆组成的单个环状重叠群构成，沿其排列有211个标记。其中100个序列标记与已知基因有很强的相似性。许多重叠区域也通过限制性指纹分析进行了检查。该图谱是阐明嗜热嗜酸栖热菌全基因组序列的重要一步。为此，我们用15000个随机序列确定了超过95%的基因组。每个序列都已在公共序列数据库中进行筛选，以确定与已知基因的相似性。我们在此报告已鉴定出的474个推定基因的列表。
Zotero 插件