Malkawi H I, Youssef M T
Department of Biological Sciences, Yarmouk University, Irbid-Jordan.
J Trop Pediatr. 1998 Jun;44(3):128-32. doi: 10.1093/tropej/44.3.128.
The antibiotic resistance pattern and plasmid profiles were studied for a total of 89 Escherichia coli (E. coli) isolates recovered from faecal specimens of children hospitalized with diarrhoea in Rahma hospital for children, Irbid-Jordan. Twenty-four of these were identified as diarrhoeagenic E. coli by the polymerase chain reaction (PCR) procedure. Seventy-one E. coli isolates showed a multiple resistance pattern (to 10 antibiotics) and 13 isolates were susceptible to all antibiotics tested. All the 89 isolates were sensitive to nalidixic acid. Plasmid content and profile studies showed that the 76 E. coli drug resistant isolates (including 6 and 12 isolates that showed single resistance to tetracycline and ampicillin respectively) carried plasmids ranging from 54.0 kb to 1.5 kb in size. The number of plasmids in each of these isolates ranged from one to six of different sizes. All the E. coli isolates contained a common plasmid with a molecular weight of 25.0 kb which proved to confer resistance to ampicillin, kanamycin, tetracycline, and possibly chloramphenicol.
对从约旦伊尔比德拉赫马儿童医院腹泻住院儿童粪便标本中分离出的89株大肠杆菌进行了抗生素耐药模式和质粒图谱研究。其中24株通过聚合酶链反应（PCR）程序被鉴定为致泻性大肠杆菌。71株大肠杆菌分离株表现出多重耐药模式（对10种抗生素耐药），13株分离株对所有测试抗生素敏感。所有89株分离株对萘啶酸敏感。质粒含量和图谱研究表明，76株耐药大肠杆菌分离株（包括分别对四环素和氨苄西林表现出单一耐药性的6株和12株分离株）携带大小从54.0 kb到1.5 kb不等的质粒。这些分离株中每株的质粒数量从1个到6个不同大小不等。所有大肠杆菌分离株都含有一个分子量为25.0 kb的共同质粒，该质粒被证明可赋予对氨苄西林、卡那霉素、四环素以及可能对氯霉素的耐药性。
