Steger K, Tetens F, Seitz J, Grothe C, Bergmann M
Institute of Anatomy and Cell Biology, University of Halle, Germany.
Histochem Cell Biol. 1998 Jul;110(1):57-62. doi: 10.1007/s004180050265.
Fibroblast growth factor 2 (FGF-2), which occurs in various isoforms both species and tissue specifically, regulates cell proliferation and differentiation via a dual receptor system consisting of heparan sulphate proteoglycans and receptor tyrosine kinases (FGFRs). This study demonstrates for the first time the distribution pattern of FGF-2 and the receptors FGFR 1-4 in the normal seminiferous epithelium of adult men. In western blot analyses, the polyclonal antibody, anti-FGF-2, shows two immunoreactive bands at 18 and 24 kDa. On paraffin sections, positive immunoreaction occurs within the cytoplasm of spermatogonia. The distribution pattern of the polyclonal anti-FGFR 1-4 antibodies is as follows: anti-FGFR-1 (one 68-kDa band) stains nuclei and cytoplasm of spermatogonia; anti-FGFR-3 (five bands at 68, 78, 105, 125 and 145 kDa) stains the nuclei of all germ cells except those of elongated spermatids; and anti-FGFR-4 (one 48-kDa band) stains the cytoplasm of primary pachytene spermatocytes. We were unable to demonstrate FGFR-2 immunoreactivity either in western blot analysis or on paraffin sections. This distribution pattern suggests that FGF-2 in spermatogonia is involved in the autocrine and paracrine regulation of the proliferation and differentiation of spermatogonia and spermatocytes via the receptors FGFR-1, FGFR-3 and FGFR-4.
成纤维细胞生长因子2（FGF - 2）以物种和组织特异性的多种同工型存在，通过由硫酸乙酰肝素蛋白聚糖和受体酪氨酸激酶（FGFRs）组成的双受体系统调节细胞增殖和分化。本研究首次展示了FGF - 2和受体FGFR 1 - 4在成年男性正常生精上皮中的分布模式。在蛋白质印迹分析中，多克隆抗体抗FGF - 2在18 kDa和24 kDa处显示出两条免疫反应带。在石蜡切片上，精原细胞的细胞质内出现阳性免疫反应。多克隆抗FGFR 1 - 4抗体的分布模式如下：抗FGFR - 1（一条68 kDa带）对精原细胞核对和细胞质染色；抗FGFR - 3（68、78、105、125和145 kDa处的五条带）对除伸长型精子细胞外的所有生殖细胞的细胞核染色；抗FGFR - 4（一条48 kDa带）对初级粗线期精母细胞的细胞质染色。在蛋白质印迹分析或石蜡切片上，我们均未证实FGFR - 2的免疫反应性。这种分布模式表明，精原细胞中的FGF - 2通过受体FGFR - 哪1、FGFR - 3和FGFR - 4参与精原细胞和精母细胞增殖与分化的自分泌和旁分泌调节。
