Svenningsson P, Nergårdh R, Fredholm B B
Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden.
Neuropharmacology. 1998;37(3):331-7. doi: 10.1016/s0028-3908(98)00045-8.
By using in situ hybridisation we examined the acute effects of caffeine on haloperidol-induced c-fos mRNA in rat striatum. A homogeneous induction of striatal c-fos mRNA was found 30 min after injection of haloperidol (1 mg kg(-1)). At this timepoint caffeine (40 mg kg(-1)) did not affect c-fos mRNA in striatum but caused a significant increase of this gene in the somatosensory cortex. When caffeine was injected together with haloperidol c-fos mRNA was reduced in the medial part of the striatum, but enhanced in the caudal part. Similar region-specific effects of caffeine were observed on c-fos mRNA induced by the selective dopamine D2 antagonist raclopride (0.5 mg kg(-1)). Both haloperidol and raclopride counteracted caffeine-induced c-fos mRNA expression in somatosensory cortex. By contrast no significant interactions between caffeine and the dopamine D1 antagonist SCH 23390 (0.5 mg kg(-1)) on striatal c-fos mRNA expression were observed. The present data show that caffeine modulates c-fos mRNA induced by dopamine D2 receptor antagonism differentially in sensorimotor and limbic-related areas of striatum. It is suggested that this could depend upon a different action of caffeine on the cortical inputs to these two parts of the striatum.
我们运用原位杂交技术研究了咖啡因对大鼠纹状体中氟哌啶醇诱导的c-fos mRNA的急性影响。注射氟哌啶醇（1 mg kg⁻¹）30分钟后，纹状体c-fos mRNA出现均匀诱导。此时，咖啡因（40 mg kg⁻¹）对纹状体中的c-fos mRNA没有影响，但导致体感皮层中该基因显著增加。当咖啡因与氟哌啶醇一起注射时，纹状体内侧部分的c-fos mRNA减少，但在尾部增强。在选择性多巴胺D2拮抗剂雷氯必利（0.5 mg kg⁻¹）诱导的c-fos mRNA上也观察到咖啡因类似的区域特异性作用。氟哌啶醇和雷氯必利都抵消了咖啡因在体感皮层诱导的c-fos mRNA表达。相比之下，未观察到咖啡因与多巴胺D1拮抗剂SCH 23390（0.5 mg kg⁻¹）对纹状体c-fos mRNA表达有显著相互作用。目前的数据表明，咖啡因在纹状体的感觉运动和边缘相关区域对多巴胺D2受体拮抗诱导的c-fos mRNA有不同的调节作用。有人认为，这可能取决于咖啡因对这两部分纹状体皮质输入的不同作用。
