Holzgrabe U, Diehl B W, Wawer I
Pharmazeutisches Institut, Universität Bonn, Germany.
J Pharm Biomed Anal. 1998 Aug;17(4-5):557-616. doi: 10.1016/s0731-7085(97)00276-8.
Since drugs in clinical use are mostly synthetic or natural products, NMR spectroscopy has been mainly used for the elucidation and confirmation of structures. For the last decade, NMR methods have been introduced to quantitative analysis in order to determine the impurity profile of a drug, to characteristic the composition of drug products, and to investigate metabolites of drugs in body fluids. For pharmaceutical technologists, solid state measurements can provide information about polymorphism of drug powders, conformation of drugs in tablets etc. Micro-imaging can be used to study the dissolution of tablets, and whole-body imaging is a powerful tool in clinical diagnostics. Taken together, this review covers applications of NMR spectroscopy in drugs analysis, in particular, methods of international pharmacopoeiae, pharmaceutics and pharmacokinetics. The authors have repeated many of the methods describe in their own laboratories.
由于临床使用的药物大多是合成或天然产物，核磁共振光谱法主要用于结构的阐明和确认。在过去十年中，核磁共振方法已被引入定量分析，以确定药物的杂质概况、表征药品的组成以及研究体液中药物的代谢产物。对于制药技术人员来说，固态测量可以提供有关药物粉末多晶型、片剂中药物构象等信息。微观成像可用于研究片剂的溶解情况，而全身成像则是临床诊断中的有力工具。综上所述，本综述涵盖了核磁共振光谱法在药物分析中的应用，特别是国际药典、药剂学和药代动力学方面的方法。作者在自己的实验室中重复了许多所描述的方法。
