Sadeghipour F, Veuthey J L
Laboratoire de Chimie Analytique Pharmaceutique, Université de Genève, Switzerland.
J Pharm Biomed Anal. 1998 Aug;17(4-5):801-10. doi: 10.1016/s0731-7085(98)00012-0.
An automated on-line dialysis coupled to a trace enrichment method has been developed for the separation and quantification of four methylenedioxylated amphetamines in serum and plasma, using liquid chromatography coupled to a fluorimetric detector. The on-line dialysis method was optimized and validated on fresh human serum and plasma samples. This sample preparation method allowed the quantification of methylenedioxylated amphetamines in serum or plasma, at concentrations as low as ca. 10 ng ml-1, with good repeatability, reproducibility and accuracy. The automated on-line dialysis method took less than 30 min. This method was applied to seven toxicological cases and results showed that the concentration of methylenedioxylated amphetamines in blood was in the range of 20-484 ng ml-1.
已开发出一种与痕量富集方法联用的自动化在线透析方法，用于血清和血浆中四种亚甲二氧基苯丙胺的分离和定量分析，采用液相色谱与荧光检测器联用。在线透析方法在新鲜人血清和血浆样本上进行了优化和验证。这种样品制备方法能够对血清或血浆中的亚甲二氧基苯丙胺进行定量分析，最低检测浓度约为10 ng/ml，具有良好的重复性、重现性和准确性。自动化在线透析方法耗时不到30分钟。该方法应用于7例毒理学病例，结果表明血液中亚甲二氧基苯丙胺的浓度在20 - 484 ng/ml范围内。
