Yamada H, Honda T, Kikuchi S, Sugiura Y
Department of Orthopaedic Surgery, Fukushima Medical University School of Medicine, Japan.
Spine (Phila Pa 1976). 1998 Jul 15;23(14):1524-9; discussion 1529-30. doi: 10.1097/00007632-199807150-00004.
Sensory innervation in the cervical dura mater of rats was investigated immunohistochemically in whole tissues and transverse sections of the decalcified vertebral column.
To investigate the origin and distribution of sensory innervation in the cervical dura mater.
It has been generally accepted that irritation of the cervical structures is one of the major causes of pain in the neck and the upper extremities. Sensory fibers in the cervical dura mater are possible mediators of pain. However, there is little information about sensory innervation in the cervical dura mater, including the epiradicular sheath.
Ten Wistar rats were used for wholemount immunohistochemical observations of the cervical dura mater. The vertebral columns of five rats were processed for immunohistochemistry after decalcification. In all specimens, sensory fibers were demonstrated by the peptide immunohistochemistry, and sensory innervation was examined.
The cervical dura mater was arbitrarily divided into three areas: ventral, dorsal, dorsal root ganglion. A large number of fibers were in the dorsal root ganglion area and were distributed in the corresponding segments. Some calcitonin gene-related peptide immunoreactive fibers in the dorsal root ganglion were directly innervated from dorsal root ganglion area neurons and did not form nerve bundles, similar to the sinuvertebral nerve. Several immunoreactive fibers were seen in the ventral area; fibers were rarely observed in the dorsal area.
A large number of sensory fibers are segmentally distributed in the cervical dura mater, and some of them are directly traced from dorsal root ganglion neurons.
采用免疫组织化学方法，在大鼠颈椎硬脊膜的全组织和脱钙脊柱横切面上研究感觉神经支配。
研究颈椎硬脊膜感觉神经支配的起源和分布。
一般认为，颈椎结构受刺激是颈部和上肢疼痛的主要原因之一。颈椎硬脊膜中的感觉纤维可能是疼痛的介质。然而，关于颈椎硬脊膜包括根上鞘的感觉神经支配的信息很少。
选用10只Wistar大鼠对颈椎硬脊膜进行整装免疫组织化学观察。5只大鼠的脊柱经脱钙后进行免疫组织化学处理。在所有标本中，通过肽免疫组织化学显示感觉纤维，并检查感觉神经支配。
颈椎硬脊膜被任意分为三个区域：腹侧、背侧、背根神经节。大量纤维位于背根神经节区域，并分布于相应节段。背根神经节中一些降钙素基因相关肽免疫反应性纤维直接由背根神经节区域的神经元支配，不形成神经束，类似于窦椎神经。腹侧区域可见一些免疫反应性纤维；背侧区域很少观察到纤维。
大量感觉纤维节段性分布于颈椎硬脊膜，其中一些直接起源于背根神经节神经元。
