Shimizu Toshihiko, Toriumi Haruki, Sato Hideki, Shibata Mamoru, Nagata Eiichiro, Gotoh Kyoko, Suzuki Norihiro
Department of Neurology, School of Medicine, Keio University, 35 Shinanomachi, Tokyo, 160-8582, Japan.
Brain Res. 2007 Oct 10;1173:84-91. doi: 10.1016/j.brainres.2007.07.068. Epub 2007 Aug 10.
We examined the distribution and origin of the nerve fibers innervating the dura mater of the rat that show immunoreactivity for the TRPV1 receptor (TRPV1-IR). Nearly 70% of the nerve fibers showing TRPV1-IR in the dura mater also exhibited CGRP-IR. Using a combination of immunohistochemistry and a retrograde tracer technique, we detected tracer accumulation in 0.6% of the neurons in the trigeminal ganglion and a few neurons in the dorsal root ganglion; half of the neurons in the trigeminal ganglion were small- and medium-sized (<or=1000 microm2). Among the tracer-accumulated neurons in the trigeminal ganglion, approximately 25% exhibited TRPV1-IR. Furthermore, nearly 80% of the tracer-accumulated small- and medium-sized neurons in the trigeminal ganglion that exhibited TRPV1-IR also exhibited CGRP-IR. Our findings indicate that the TRPV1 receptor in the dura mater and sensory ganglia may contribute to the pathophysiology of migraine, providing an important clue for the development of therapeutic strategies for migraine.
我们研究了大鼠硬脑膜中对TRPV1受体呈免疫反应性（TRPV1-IR）的神经纤维的分布和起源。硬脑膜中显示TRPV1-IR的神经纤维近70%也表现出降钙素基因相关肽免疫反应性（CGRP-IR）。通过免疫组织化学和逆行示踪技术相结合，我们在三叉神经节0.6%的神经元以及背根神经节的少数神经元中检测到示踪剂积聚；三叉神经节中一半的神经元为中小尺寸（≤1000平方微米）。在三叉神经节中示踪剂积聚的神经元中，约25%表现出TRPV1-IR。此外，三叉神经节中示踪剂积聚且表现出TRPV1-IR的中小尺寸神经元近80%也表现出CGRP-IR。我们的研究结果表明，硬脑膜和感觉神经节中的TRPV1受体可能与偏头痛的病理生理学有关，为偏头痛治疗策略的开发提供了重要线索。
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