Waber-Wenger Barbara, Forterre Franck, Kuehni-Boghenbor Kathrin, Danuser Renzo, Stein Jens Volker, Stoffel Michael Hubert
Division of Veterinary Anatomy, Vetsuisse Faculty, University of Bern, Länggass-Strasse 120, POB 8466, 3001, Bern, Switzerland.
Histochem Cell Biol. 2014 Oct;142(4):433-47. doi: 10.1007/s00418-014-1218-x. Epub 2014 Apr 20.
Although intervertebral disc herniation is a well-known disease in dogs, pain management for this condition has remained a challenge. The goal of the present study is to address the lack of information regarding the innervation of anatomical structures within the canine vertebral canal. Immunolabeling was performed with antibodies against protein gene product 9.5, Tuj-1 (neuron-specific class III β-tubulin), calcitonin gene-related peptide, and neuropeptide Y in combination with the lectin from Lycopersicon esculentum as a marker for blood vessels. Staining was indicative of both sensory and sympathetic fibers. Innervation density was the highest in lateral areas, intermediate in dorsal areas, and the lowest in ventral areas. In the dorsal longitudinal ligament (DLL), the highest innervation density was observed in the lateral regions. Innervation was lower at mid-vertebral levels than at intervertebral levels. The presence of sensory and sympathetic fibers in the canine dura and DLL suggests that pain may originate from both these structures. Due to these regional differences in sensory innervation patterns, trauma to intervertebral DLL and lateral dura is expected to be particularly painful. The results ought to provide a better basis for the assessment of medicinal and surgical procedures.
虽然椎间盘突出症在犬类中是一种广为人知的疾病，但针对这种病症的疼痛管理仍然是一项挑战。本研究的目的是解决关于犬椎管内解剖结构神经支配信息的不足。使用针对蛋白基因产物9.5、Tuj-1（神经元特异性III类β-微管蛋白）、降钙素基因相关肽和神经肽Y的抗体，并结合来自番茄的凝集素作为血管标记物进行免疫标记。染色显示出感觉纤维和交感神经纤维。神经支配密度在外侧区域最高，背侧区域次之，腹侧区域最低。在背侧纵韧带（DLL）中，外侧区域的神经支配密度最高。椎体中部水平的神经支配低于椎间盘水平。犬硬脑膜和DLL中存在感觉纤维和交感神经纤维，这表明疼痛可能源于这两种结构。由于感觉神经支配模式存在这些区域差异，预计椎间盘DLL和外侧硬脑膜的创伤会特别疼痛。这些结果应该为评估药物和手术治疗提供更好的依据。
