Nishimura K, Mochida J
Department of Orthopaedic Surgery, Tokai University School of Medicine, Kanagawa, Japan.
Spine (Phila Pa 1976). 1998 Jul 15;23(14):1531-8; discussion 1539. doi: 10.1097/00007632-199807150-00006.
An analysis of the histologic changes in intervertebral discs after percutaneous reinsertion of the nucleus pulposus in rats.
To devise a way to delay further disc degeneration resulting from spinal deformity and the adverse effects of various treatments.
The role of the nucleus pulposus of the intervertebral disc described by many investigators has not been fully clarified.
Disc herniation was induced in the tails of 112 Wistar rats, using a fixation device between the 5th and 8th coccygeal vertebrae. After percutaneous nucleotomy at coccygeal vertebrae 5-6 and 6-7, fresh nucleus pulposus, cryopreserved nucleus pulposus, or an artificial substitute was inserted into the intervertebral disc at coccygeal vertebrae 5-6. Two, 4, or 8 weeks after reinsertion, disc sections from each coccygeal level were studied histopathologically.
In the groups with reinsertion of fresh or cryopreserved nucleus pulposus, degenerative changes of the disc with the reinserted nucleus at coccygeal vertebrae 5-6 were milder than those of the disc without reinsertion at coccygeal vertebrae 6-7. However, no apparent benefit from reinsertion was observed in the group with artificial substitutes.
Early reinsertion of the nucleus pulposus (fresh or cryopreserved) delays degeneration of such disc materials as the anulus fibrosus, endplate, and remaining nucleus pulposus.
对大鼠经皮再植入髓核后椎间盘组织学变化的分析。
设计一种方法来延缓因脊柱畸形和各种治疗的不良反应导致的椎间盘进一步退变。
许多研究者所描述的椎间盘髓核的作用尚未完全阐明。
使用第5至第8尾椎之间的固定装置，在112只Wistar大鼠的尾部诱发椎间盘突出。在第5 - 6和第6 - 7尾椎进行经皮髓核摘除术后，将新鲜髓核、冷冻保存的髓核或人工替代物植入第5 - 6尾椎的椎间盘。再植入后2、4或8周，对每个尾椎水平的椎间盘切片进行组织病理学研究。
在再植入新鲜或冷冻保存髓核的组中，第5 - 6尾椎再植入髓核的椎间盘退变变化比第6 - 7尾椎未再植入髓核的椎间盘轻。然而，在使用人工替代物的组中未观察到再植入的明显益处。
早期再植入髓核（新鲜或冷冻保存）可延缓纤维环、终板和剩余髓核等椎间盘组织的退变。
