Mert Ahmet, Ikinci Keles Ayse, Aydin Murat, Erol Huseyin Serkan, Sonmez Osman Fikret
Department of Orthopedics and Traumatology, Private Pendik Yüzyıl Hospital, Istanbul, Türkiye.
Department of Histology and Embryology, Aksaray University, Faculty of Medicine, Aksaray, Türkiye.
Front Bioeng Biotechnol. 2025 Jun 18;13:1613148. doi: 10.3389/fbioe.2025.1613148. eCollection 2025.
This study aimed to investigate the regenerative effects of PRP on an experimental rat model of disc degeneration using histological and biochemical parameters. Additionally, we evaluated whether ropivacaine, a local anesthetic commonly used in clinical practice, affects the efficacy of PRP.
Rats were randomly divided into five groups as control and treatment groups. Disc degeneration models were established using appropriate procedures. On the intervention day, PRP was prepared from whole blood collected from the rats. PRP, PRP + ropivacaine, or ropivacaine alone was administered at the appropriate doses and according to standardized protocols.
In the untreated disc degeneration groups, annulus fibrosus (AF) and nucleus pulposus (NP) boundaries were indistinct, disc morphology was disrupted, collagen structures in the AF were degenerated or irregular, and vacuolization, interstitial edema, and necrotic tissue remnants were observed in the NP region. In contrast, in groups treated with PRP and PRP + ropivacaine, a reduction in edema and vacuolization, disappearance of necrotic tissue, restoration of distinct NP and AF boundaries, and decreased atrophy and cellular clustering in NP cells were observed. Biochemical analysis showed that IL-6 and TNF-α levels were within normal ranges in the groups treated with PRP and PRP + ropivacaine, whereas these levels remained elevated in the untreated disc degeneration groups, indicating ongoing effects of degeneration.
This study demonstrates the regenerative effects of PRP in disc degeneration through histological and biochemical parameters. Furthermore, the addition of ropivacaine to PRP did not exert any negative effects on PRP's regenerative properties.
本研究旨在利用组织学和生化参数，研究富血小板血浆（PRP）对椎间盘退变实验大鼠模型的再生作用。此外，我们评估了临床实践中常用的局部麻醉药罗哌卡因是否会影响PRP的疗效。
将大鼠随机分为五组，即对照组和治疗组。采用适当程序建立椎间盘退变模型。在干预日，从大鼠采集的全血中制备PRP。以适当剂量并根据标准化方案给予PRP、PRP+罗哌卡因或单独的罗哌卡因。
在未治疗的椎间盘退变组中，纤维环（AF）和髓核（NP）边界不清晰，椎间盘形态破坏，AF中的胶原结构退变或不规则，并且在NP区域观察到空泡化、间质水肿和坏死组织残余。相比之下，在接受PRP和PRP+罗哌卡因治疗的组中，观察到水肿和空泡化减轻、坏死组织消失、NP和AF边界恢复清晰，以及NP细胞萎缩和细胞聚集减少。生化分析表明，PRP和PRP+罗哌卡因治疗组的白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）水平在正常范围内，而在未治疗的椎间盘退变组中这些水平仍然升高，表明退变仍在持续。
本研究通过组织学和生化参数证明了PRP在椎间盘退变中的再生作用。此外，在PRP中添加罗哌卡因对PRP的再生特性没有产生任何负面影响。
