Papadopoulou G, Karagouni E, Dotsika E
Hellenic Pasteur Institute, Athens, Greece.
Vaccine. 1998 May-Jun;16(9-10):885-92. doi: 10.1016/s0264-410x(97)00308-3.
The major surface glycoprotein (gp63) of Leishmania major incorporated into the immunostimulating complexes (ISCOMs) was used to protect Balb/c mice against experimental infection. Two intraperitoneal vaccinations with low doses of gp63 into ISCOMs (gp63-ISCOMs) induced protective immunity in vaccinated mice as indicated by reduced inflammation and suppressed lesions after experimental challenge. An augmented IgG-specific secretion and a specific switching towards the IgG2a isotype was observed in the serum of vaccinated mice. Gp63-ISCOMs primed spleen cells restimulated in vitro with soluble Leishmania antigen (SLA) or live parasites displayed strong gp63-specific proliferative responses and secreted high levels of interleukin-2, interferon gamma and interleukin-10 but not interleukin-4. No delayed type hypersensitivity response to either SLA or LV39 was detected. These data indicate that gp63-ISCOMs induced a protective immunity in the susceptible Balb/c mice against Leishmania challenge, modulating the immune response towards a Th1 rather than Th2 type.
利什曼原虫主要表面糖蛋白（gp63）被整合到免疫刺激复合物（ISCOMs）中，用于保护Balb/c小鼠免受实验性感染。两次低剂量的gp63腹腔注射到ISCOMs（gp63-ISCOMs）中，可诱导接种小鼠产生保护性免疫，实验攻击后炎症减轻和病变受到抑制即表明了这一点。在接种小鼠血清中观察到IgG特异性分泌增加以及向IgG2a亚型的特异性转换。用可溶性利什曼原虫抗原（SLA）或活寄生虫在体外再次刺激经gp63-ISCOMs致敏的脾细胞，显示出强烈的gp63特异性增殖反应，并分泌高水平的白细胞介素-2、干扰素γ和白细胞介素-10，但不分泌白细胞介素-4。未检测到对SLA或LV39的迟发型超敏反应。这些数据表明，gp63-ISCOMs在易感的Balb/c小鼠中诱导了针对利什曼原虫攻击的保护性免疫，将免疫反应调节为Th1型而非Th2型。
