Aasted B, Alexandersen S, Christensen J
Department of Veterinary Microbiology, Royal Veterinary and Agricultural University, Copenhagen, Denmark.
Vaccine. 1998 Jul;16(11-12):1158-65. doi: 10.1016/s0264-410x(98)80114-x.
Vaccination studies were performed with partially purified recombinant AMDV VP1/2 capsids as well as with the major AMDV non-structural protein (NS1). All vaccine constructs induced an antibody response, but did not prevent infection upon challenge with AMDV. The severity of Aleutian disease (AD) was judged by the serum gammaglobulin level, the quantity of peripheral blood CD8 lymphocytes, antibody titers to VP1/2 and NS1 proteins and mink death rates. The VP1/2 vaccine constructs enhanced the disease process with drastic death rates for the vaccinated mink. On the contrary, the NS1 vaccine constructs resulted in milder AD than seen in the non-vaccinated mink.
使用部分纯化的重组水貂阿留申病病毒（AMDV）VP1/2衣壳以及主要的AMDV非结构蛋白（NS1）进行了疫苗接种研究。所有疫苗构建体均诱导了抗体反应，但在受到AMDV攻击时并不能预防感染。通过血清γ球蛋白水平、外周血CD8淋巴细胞数量、针对VP1/2和NS1蛋白的抗体滴度以及水貂死亡率来判断阿留申病（AD）的严重程度。VP1/2疫苗构建体加剧了疾病进程，接种疫苗的水貂死亡率极高。相反，NS1疫苗构建体导致的AD比未接种疫苗的水貂更轻。
