牛朊蛋白基因的基因组结构及牛朊蛋白cDNA的完整核苷酸序列。

Genomic structure of the bovine PrP gene and complete nucleotide sequence of bovine PrP cDNA.

作者信息

Horiuchi M, Ishiguro N, Nagasawa H, Toyoda Y, Shinagawa M

机构信息

Department of Veterinary Public Health, University of Agriculture and Veterinary Medicine, Obihiro, Japan.

出版信息

Anim Genet. 1998 Feb;29(1):37-40. doi: 10.1046/j.1365-2052.1998.00250.x.

DOI:10.1046/j.1365-2052.1998.00250.x

PMID:9682448

Abstract

The present authors previously reported the nucleotide sequence of the 5' half of a cDNA encoding bovine prion protein (PrP) and the genomic structure of the bovine PrP gene encoding the 5' -untranslated region. Here they report the extent of intron 2 of the bovine PrP gene and the nucleotide sequence of the 3' half of bovine PrP cDNA that had not been determined before. This newly sequenced 3' half of the bovine PrP cDNA consisted of 2149 bp. The entire 3'-untranslated region (3'-UTR) was found to be encoded by a single exon, exon 3. One nucleotide polymorphism was found in the 3'-UTR. The length of intron 2 was estimated to be about 14 kbp. The structure of bovine PrP gene can be defined by combining the present results and previous reports on the bovine PrP gene.

摘要

本研究作者先前报道了编码牛朊病毒蛋白（PrP）的cDNA 5'端一半的核苷酸序列以及编码5'非翻译区的牛PrP基因的基因组结构。在此，他们报道了牛PrP基因内含子2的范围以及之前未确定的牛PrP cDNA 3'端一半的核苷酸序列。新测序的牛PrP cDNA的这一3'端一半由2149个碱基对组成。整个3'非翻译区（3'-UTR）由单个外显子，即外显子3编码。在3'-UTR中发现了一个单核苷酸多态性。内含子2的长度估计约为14千碱基对。通过结合本研究结果和先前关于牛PrP基因的报道，可以确定牛PrP基因的结构。