大鼠内耳中血管活性肠肽（VIP）及其受体的mRNA的存在。

Presence of mRNA for vasoactive intestinal polypeptide (VIP) and its receptor in the rat inner ear.

作者信息

Kitano H, Takeda T, Suzuki M, Kitanishi T, Yazawa Y, Kitajima K, Kimura H, Tooyama I

机构信息

Department of Otolaryngology, Shiga University of Medical Science, Otsu, Japan.

出版信息

Hear Res. 1998 Jul;121(1-2):109-11. doi: 10.1016/s0378-5955(98)00070-7.

DOI:10.1016/s0378-5955(98)00070-7

PMID:9682813

Abstract

Although mechanisms regulating inner ear fluid have not been yet elucidated, control of blood flow has been thought to be of great importance. Vasoactive intestinal polypeptide (VIP) was the first neuropeptide demonstrated in cerebrovascular nerves. To study the possible role of VIP in regulation of inner ear fluid, we investigated the presence of mRNA for VIP and VIP receptor in the rat inner ear using a reverse transcription-polymerase chain reaction (RT-PCR) method. A single band of the size expected for VIP and its receptor was detected in mRNA from the rat inner ear by using primers specific for VIP and the receptor. The nucleotide sequences of the subcloned RT-PCR products were identical to those of rat VIP and the rat lung VIP receptor. These results indicate that both VIP and VIP receptor are expressed in the inner ear of the rat and suggest that VIP may be implicated in regulation of fluid in the inner ear.

摘要

尽管调节内耳液体的机制尚未阐明，但人们认为控制血流非常重要。血管活性肠肽（VIP）是在脑血管神经中发现的首个神经肽。为了研究VIP在调节内耳液体中的可能作用，我们使用逆转录聚合酶链反应（RT-PCR）方法，研究了大鼠内耳中VIP及其受体的mRNA的存在情况。通过使用针对VIP及其受体的特异性引物，在大鼠内耳的mRNA中检测到了预期大小的VIP及其受体的单一条带。亚克隆的RT-PCR产物的核苷酸序列与大鼠VIP和大鼠肺VIP受体的序列相同。这些结果表明，VIP及其受体均在大鼠内耳中表达，提示VIP可能参与内耳液体的调节。