大鼠腹膜淋巴细胞和巨噬细胞中血管活性肠肽（VIP）及VIP1受体基因表达的特征分析

Characterization of gene expression of VIP and VIP1-receptor in rat peritoneal lymphocytes and macrophages.

作者信息

Delgado M, Pozo D, Martinez C, Garrido E, Leceta J, Calvo J R, Gomariz R P

机构信息

Departamento de Biología Celular, Facultad de Biología, Universidad Complutense, Madrid, Spain.

出版信息

Regul Pept. 1996 Apr 23;62(2-3):161-6. doi: 10.1016/0167-0115(96)00024-9.

DOI:10.1016/0167-0115(96)00024-9

PMID:8795080

Abstract

In the present report we show the gene expression pattern of VIP and VIP1 receptor in two peritoneal cell populations, macrophages and lymphocytes by reverse transcription (RT) and polymerase chain reaction (PCR). Only in the lymphoid cells we have obtained a specific VIP cDNA product of 458 bp identical in size to the one obtained from cerebral cortex. On the other hand, we have obtained in both peritoneal populations lymphocytes and macrophages, a specific VIP1 receptor cDNA product of 311 bp identical in size to that obtained from lung. These results have been confirmed by Southern blot hybridization. Our findings suggest an autocrine/paracrine action of VIP in peritoneal microenvironment, supporting an immunoregulatory role for this neuropeptide.

摘要

在本报告中，我们通过逆转录（RT）和聚合酶链反应（PCR）展示了血管活性肠肽（VIP）及其1型受体（VIP1受体）在两种腹膜细胞群体（巨噬细胞和淋巴细胞）中的基因表达模式。仅在淋巴细胞中，我们获得了一个458 bp的特异性VIP cDNA产物，其大小与从大脑皮层获得的产物相同。另一方面，我们在腹膜细胞群体中的淋巴细胞和巨噬细胞中均获得了一个311 bp的特异性VIP1受体cDNA产物，其大小与从肺中获得的产物相同。这些结果已通过Southern印迹杂交得到证实。我们的发现表明VIP在腹膜微环境中具有自分泌/旁分泌作用，支持了这种神经肽的免疫调节作用。