Cobbs C S, Chen J, Greenberg D A, Graham S H
Department of Neurosurgery, University of Alabama, Birmingham, USA.
Neurosci Lett. 1998 Jun 19;249(2-3):79-82. doi: 10.1016/s0304-3940(98)00377-2.
Vascular endothelial growth factor (VEGF) has been implicated in hypoxia-induced angiogenesis in tumors and ischemia. We examined VEGF mRNA and protein expression after occlusion of the middle cerebral artery (MCA) in rats. VEGF mRNA expression studied by in situ hybridization was increased in the ischemic border zone 24 h after 30, 60 or 120 min of focal cerebral ischemia. VEGF protein expression measured by Western blots was also increased in this region 24 and 48 h after ischemia, and VEGF immunocytochemistry localized this increased expression to astroglia. Thus, VEGF is induced after focal cerebral ischemia and could have a role in pathophysiology and recovery in the ischemic border zone.
血管内皮生长因子（VEGF）与肿瘤和局部缺血中缺氧诱导的血管生成有关。我们检测了大鼠大脑中动脉（MCA）闭塞后VEGF mRNA和蛋白的表达。通过原位杂交研究发现，局灶性脑缺血30、60或120分钟后24小时，缺血边缘区VEGF mRNA表达增加。通过蛋白质印迹法检测发现，缺血后24小时和48小时该区域VEGF蛋白表达也增加，并且VEGF免疫细胞化学将这种增加的表达定位于星形胶质细胞。因此，局灶性脑缺血后可诱导VEGF表达，其可能在缺血边缘区的病理生理过程和恢复中发挥作用。
