Stahel P F, Kossmann T, Joller H, Trentz O, Morganti-Kossmann M C
Department of Surgery, University Hospital, Zürich, Switzerland.
Neurosci Lett. 1998 Jun 19;249(2-3):123-6. doi: 10.1016/s0304-3940(98)00410-8.
An overwhelming intracranial inflammatory response occurs as a consequence of severe head trauma, leading to cerebral edema and secondary brain injury. Cytokines are important mediators of post-traumatic cerebral inflammation. In the present study, levels of interleukin-12 (IL-12), a pro-inflammatory cytokine which activates cellular immune response mechanisms, were measured by ELISA in 140 matched serum and ventricular cerebrospinal fluid (CSF) samples from ten patients with severe traumatic brain injury. The mean IL-12 CSF levels were significantly elevated in all patients in the course of 14 days after trauma, compared to CSF samples from 15 control patients. Assessment of the IL-12 CSF/serum ratio and of the blood-brain barrier function, using the CSF/serum albumin ratio, suggest that elevated IL-12 CSF levels might be in part derived from intracerebral cytokine synthesis.
严重头部创伤会引发强烈的颅内炎症反应，导致脑水肿和继发性脑损伤。细胞因子是创伤后脑炎症的重要介质。在本研究中，通过酶联免疫吸附测定法（ELISA）检测了10例重度创伤性脑损伤患者的140份配对血清和脑室脑脊液（CSF）样本中白细胞介素-12（IL-12）的水平，IL-12是一种激活细胞免疫反应机制的促炎细胞因子。与15例对照患者的脑脊液样本相比，所有患者在创伤后14天内脑脊液中IL-12的平均水平均显著升高。使用脑脊液/血清白蛋白比率评估IL-12脑脊液/血清比率和血脑屏障功能，结果表明脑脊液中IL-12水平升高可能部分源自脑内细胞因子的合成。
