Goto A, Yamada K
Second Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Clin Exp Hypertens. 1998 Jul-Aug;20(5-6):551-6. doi: 10.3109/10641969809053233.
We detected two candidates for endogenous digitalis-like factors in human urine based on the inhibition of 3H-ouabain binding to human erythrocytes. Two ouabain-displacing compound(ODC)s were consistently eluted off the C18 reverse phase HPLC column with 18% and 31% acetonitrile. The more-polar ODC-1 was ubiquitously found in mammals, markedly increased after acute and chronic salt loading in humans, and was thought to be a natriuretic factor with vasoactive property. ODC-1 mostly resembled ouabain in biological, physicochemical, and chromatographic properties and may correspond to ouabainlike compound purified by other investigators. The less-polar ODC-2 was indistinguishable from digoxin in proton nuclear magnetic resonance(NMR) and fast atom bombardment(FAB) mass spectrum.
基于对3H-哇巴因与人红细胞结合的抑制作用，我们在人尿中检测到两种内源性类洋地黄因子的候选物。两种哇巴因置换化合物（ODC）始终以18%和31%的乙腈从C18反相高效液相色谱柱上洗脱下来。极性较强的ODC-1在哺乳动物中普遍存在，在人类急性和慢性盐负荷后显著增加，被认为是一种具有血管活性的利钠因子。ODC-1在生物学、物理化学和色谱性质上与哇巴因最为相似，可能与其他研究者纯化的类哇巴因化合物相对应。极性较弱的ODC-2在质子核磁共振（NMR）和快原子轰击（FAB）质谱中与地高辛无法区分。
