Goto A, Ishiguro T, Yamada K, Ishii M, Yoshioka M, Eguchi C, Shimora M, Sugimoto T
Second Department of Internal Medicine, University of Tokyo, Japan.
Biochem Biophys Res Commun. 1990 Dec 31;173(3):1093-101. doi: 10.1016/s0006-291x(05)80898-8.
A digitalis-like factor has been purified to apparent homogeneity from human urine based on the inhibitory effect on [3H] ouabain binding to intact human erythrocytes. The purification scheme involved large scale adsorption followed by preparative, semipreparative and analytical high-performance liquid chromatography. The purified material showed a prominent digoxin-like immunoreactivity. The behaviour of the isolated substance was identical to that of authentic digoxin in three high-performance liquid chromatography and three thin-layer chromatography systems. Moreover, fast atom bombardment mass spectrum and proton nuclear magnetic resonance spectrum suggested that the purified material may be indistinguishable from digoxin.
基于对[3H]哇巴因与完整人类红细胞结合的抑制作用，已从人尿中纯化出一种类洋地黄因子，使其达到明显的均一性。纯化方案包括大规模吸附，随后进行制备型、半制备型和分析型高效液相色谱。纯化后的物质显示出显著的地高辛样免疫反应性。在三种高效液相色谱系统和三种薄层色谱系统中，分离出的物质的行为与纯品地高辛相同。此外，快原子轰击质谱和质子核磁共振谱表明，纯化后的物质可能与地高辛无法区分。
